{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T18:35:33Z","timestamp":1780684533006,"version":"3.54.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>1. The electrical properties of chemoreceptor cells from neonatal rat and adult rabbit carotid bodies (CBs) are strikingly different. These differences have been suggested to be developmental and\/or species related. To distinguish between the two possibilities, the whole\u2010cell configuration of the patch\u2010clamp technique was used to characterize the ionic currents present in isolated chemoreceptor cells from adult rat CBs. Since hypoxia\u2010induced inhibition of O2\u2010sensitive K+ currents is considered a crucial step in O2 chemoreception, the effect of hypoxia on the adult rat chemoreceptor cell currents was also studied. 2. Outward currents were carried mainly by K+, and two different components could be distinguished: a Ca(2+)\u2010dependent K+ current (IK(Ca)) sensitive to Cd2+ and charybdotoxin (CTX), and a Ca(2+)\u2010insensitive, voltage\u2010dependent K+ current (IK(V)). IK(V) showed a slow voltage\u2010dependent activation (time constant (tau) of 87.4 ms at \u201020 mV and 8.8 ms at +60 mV) and a very slow inactivation, described by the sum of two exponentials (tau 1 = 684 +\/\u2010 150 ms and tau 2 = 4.96 +\/\u2010 0.76 s at + 30 mV), that was almost voltage insensitive. The kinetic and pharmacological properties of IK(V) are typical of a delayed rectifier K+ channel. 3. Voltage\u2010dependent Ca2+ currents (ICa) were present in nineteen of twenty\u2010seven cells. TTX\u2010sensitive Na+ currents were also observed in about 10% of the cells. 4. Low PO2 (&lt; 10 mmHg) reduced the whole outward current amplitude by 22.17 +\/\u2010 1.96% (n = 27) at +20 mV. This effect was absent in the presence of Cd2+. Since low PO2 did not affect ICa, we conclude that hypoxia selectively blocks IK(Ca). 5. The properties of the currents recorded in adult rat chemoreceptor cells, including the specific inhibition of IK(Ca) by hypoxia, are similar to those reported in neonatal rat CB cells, implying that the differences between rat and rabbit chemoreceptor cells are species related.<\/jats:p>","DOI":"10.1113\/jphysiol.1997.sp021939","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T06:13:09Z","timestamp":1418796789000},"page":"429-441","source":"Crossref","is-referenced-by-count":75,"title":["Properties of ionic currents from isolated adult rat carotid body chemoreceptor cells: effect of hypoxia."],"prefix":"10.1113","volume":"499","author":[{"given":"J R","family":"L\u00f3pez-L\u00f3pez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M T","family":"P\u00e9rez-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1997,3]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1997.sp021939","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1997.sp021939","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T21:25:30Z","timestamp":1698441930000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1997.sp021939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,3]]}},"alternative-id":["10.1113\/jphysiol.1997.sp021939"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1997.sp021939","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,3]]}}}