{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T17:00:29Z","timestamp":1774976429697,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1997,5,1]],"date-time":"1997-05-01T00:00:00Z","timestamp":862444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,5]]},"abstract":"<jats:p>1. The whole\u2010cell recording mode of the patch\u2010clamp technique was used to study the effect of extracellular K+ and Rb+ on membrane currents in human osteoblasts, in a human osteoblast\u2010like cell line, and in the Jurkat human leukaemic T cell line. 2. Increasing the extracellular concentration of K+ increased the membrane conductance of the cells in a concentration\u2010dependent manner. This increase in membrane conductance was due to the activation of a Cl\u2010 conductance. Rb+ also induced this conductance, but conductance was less than half that seen in K+. 3. The Cl\u2010 channel blockers 4,4'\u2010diisothiocyanatostilbene\u20102,2'\u2010disulphonic acid (DIDS) and 4\u2010acetamido\u20104'\u2010isothiocyanatostilbene 2,2'\u2010disulphonic acid (SITS) blocked the K(+)\u2010induced Cl\u2010 current in a voltage\u2010dependent manner. The degree of blockade increased with membrane depolarization to a maximum level at 40 mV. At potentials above this value the block appeared to decrease. 4. Both tonicity and K+ were required for maximal activation of the Cl\u2010 conductance since the K(+)\u2010induced Cl\u2010 conductance could be inhibited by hypertonic solutions and the activation of a volume\u2010sensitive Cl\u2010 conductance by hypotonic solutions could be enhanced by extracellular K+. 5. We conclude that an outwardly rectifying Cl\u2010 conductance can be activated either upon osmotic swelling or by an increase in extracellular K+. Both activation pathways may be involved in cell volume regulation and seem to apply to volume\u2010sensitive Cl\u2010 channels in general since we observe this phenomenon in two different cell types, in human osteoblasts as well as in human leukaemic T lymphocytes.<\/jats:p>","DOI":"10.1113\/jphysiol.1997.sp022050","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T06:04:22Z","timestamp":1418796262000},"page":"653-660","source":"Crossref","is-referenced-by-count":14,"title":["Novel activation stimulus of chloride channels by potassium in human osteoblasts and human leukaemic T lymphocytes."],"prefix":"10.1113","volume":"500","author":[{"given":"M","family":"Steinert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Grissmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1997.sp022050","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1997.sp022050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T06:15:16Z","timestamp":1698387316000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1997.sp022050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,5]]}},"alternative-id":["10.1113\/jphysiol.1997.sp022050"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1997.sp022050","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,5]]}}}