{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:10:45Z","timestamp":1790485845146,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>Isolated interlobular ducts from the guinea\u2010pig pancreas secrete a HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>\u2010rich fluid in response to secretin. To determine the role of <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> transporters in this process, intracellular <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> concentration ([<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub>) was measured in ducts loaded with the <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>\u2010sensitive fluoroprobe, 6\u2010methoxy\u2010<jats:italic>N<\/jats:italic>\u2010ethylquinolinium chloride (MEQ). [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> decreased when the luminal <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> concentration was reduced. This effect was stimulated by forskolin, was not dependent on HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> and was not inhibited by application of the anion channel\/transporter inhibitor H<jats:sub>2<\/jats:sub>DIDS to the luminal membrane. It is therefore attributed to a cAMP\u2010stimulated <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> conductance, probably the cystic fibrosis transmembrane conductance regulator (CFTR) <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> channel. [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> also decreased when the basolateral <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> concentration was reduced. This effect was not stimulated by forskolin, was largely dependent on HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> and was inhibited by basolateral H<jats:sub>2<\/jats:sub>DIDS. It is therefore mediated mainly by <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchange. With high <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> and low HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> concentrations in the lumen, steady\u2010state [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> was 25\u201035 m<jats:sc>m<\/jats:sc> in unstimulated cells. Stimulation with forskolin caused [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> to increase by approximately 4 m<jats:sc>m<\/jats:sc> due to activation of the luminal anion exchanger. With low <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> and high HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> concentrations in the lumen to simulate physiological conditions, steady\u2010state [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> was 10\u201315 m<jats:sc>m<\/jats:sc> in unstimulated cells. Upon stimulation with forskolin, [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> fell to approximately 7 m<jats:sc>m<\/jats:sc> due to increased <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> efflux via the luminal conductance. We conclude that, during stimulation under physiological conditions, [<jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>]<jats:sub>i<\/jats:sub> decreases to very low levels in guinea\u2010pig pancreatic duct cells, largely as a result of the limited capacity of the basolateral transporters for <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> uptake. The resulting lack of competition from intracellular <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> may therefore favour HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion via anion conductances in the luminal membrane, possibly CFTR.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.012490","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"175-189","source":"Crossref","is-referenced-by-count":45,"title":["Chloride transport in microperfused interlobular ducts isolated from guinea\u2010pig pancreas"],"prefix":"10.1113","volume":"539","author":[{"given":"H.","family":"Ishiguro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Naruse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kitagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Mabuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Hayakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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