{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T07:53:17Z","timestamp":1759564397996},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>Subtype 3 of the ryanodine receptor (RYR3) is a ubiquitous Ca<jats:sup>2+<\/jats:sup> release channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain. We show by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) that non\u2010pregnant mouse myometrial cells expressed only RYR3 and therefore could be a good model for studying the role of endogenous RYR3. Expression of RYR3 was confirmed by Western blotting and immunostaining. Confocal Ca<jats:sup>2+<\/jats:sup> measurements revealed that in 1.7 m<jats:sc>m<\/jats:sc> extracellular Ca<jats:sup>2+<\/jats:sup>, neither caffeine nor photolysis of caged Ca<jats:sup>2+<\/jats:sup> were able to trigger any Ca<jats:sup>2+<\/jats:sup> responses, whereas in the same cells oxytocin activated propagated Ca<jats:sup>2+<\/jats:sup> waves. However, under conditions of increased sarcoplasmic reticulum (SR) Ca<jats:sup>2+<\/jats:sup> loading, brought about by superfusing myometrial cells in 10 m<jats:sc>m<\/jats:sc> extracellular Ca<jats:sup>2+<\/jats:sup>, all the myometrial cells responded to caffeine and photolysis of caged Ca<jats:sup>2+<\/jats:sup>, indicating that it was possible to activate RYR3. The caffeine\u2010induced Ca<jats:sup>2+<\/jats:sup> responses were inhibited by intracellular application of an anti\u2010RYR3\u2010specific antibody. Immunodetection of RYR3 with the same antibody revealed a rather homogeneous distribution of fluorescence in confocal cell sections. In agreement with these observations, spontaneous or triggered Ca<jats:sup>2+<\/jats:sup> sparks were not detected. In conclusion, our results suggest that under conditions of increased SR Ca<jats:sup>2+<\/jats:sup> loading, endogenous RYR3 may contribute to the Ca<jats:sup>2+<\/jats:sup> responses of myometrial cells.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013046","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"707-716","source":"Crossref","is-referenced-by-count":27,"title":["Identification and function of ryanodine receptor subtype 3 in non\u2010pregnant mouse myometrial 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