{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:35:05Z","timestamp":1789878905760,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>Short chain fatty acids, particularly butyrate, stimulate electroneutral <jats:styled-content>NaCl<\/jats:styled-content> absorption from the colon. Their effect in colonic epithelia lacking basal electroneutral <jats:styled-content>NaCl<\/jats:styled-content> absorption is unknown. Butyrate is also reported to inhibit active <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion in the colon. The present studies were undertaken to investigate the inter\u2010relationships between the effects of butyrate on active Na<jats:sup>+<\/jats:sup> and <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> transport in the colon. Studies were carried out in rabbit distal colon (known to have predominant electrogenic Na<jats:sup>+<\/jats:sup> absorption), rat distal colon (characterised by electroneutral Na<jats:sup>+<\/jats:sup> absorption), and hyperaldosteronaemic rat distal colon (characterised by electrogenic Na<jats:sup>+<\/jats:sup> absorption). The effect of cholera toxin (CT) was also noted. Potential difference, short\u2010circuit current (<jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>) and fluxes of Na<jats:sup>+<\/jats:sup> and <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> were measured in stripped mucosa under voltage\u2010clamp conditions. Butyrate stimulated electroneutral Na<jats:sup>+<\/jats:sup> and <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> absorption in distal colon of normal and salt\u2010depleted rats, and stimulated Na<jats:sup>+<\/jats:sup> absorption in rabbit distal colon. Amiloride (10<jats:sup>\u22124<\/jats:sup><jats:sc>m<\/jats:sc>) or CT did not inhibit this process. In rabbit distal colon, stimulation of Na<jats:sup>+<\/jats:sup> absorption by butyrate was not dependent on the presence of <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> in the medium. Butyrate significantly decreased conductance, decreased flux of sodium from serosa to mucosa (particularly in rabbit distal colon), and decreased <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>. Net <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion, induced by CT, was completely inhibited by butyrate. Stimulation of Na<jats:sup>+<\/jats:sup> absorption was independent of exposure to CT. Bumetanide reversed net <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion to net absorption, but did not alter Na<jats:sup>+<\/jats:sup> or <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> fluxes in tissues exposed to butyrate. Thus butyrate stimulates active Na<jats:sup>+<\/jats:sup> absorption in colonic epithelia, with or without expression of basal Na<jats:sup>+<\/jats:sup>\u2010H<jats:sup>+<\/jats:sup> exchange. Independently, butyrate inhibits active <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion induced by cAMP in these epithelia.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013056","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"163-173","source":"Crossref","is-referenced-by-count":38,"title":["Effects of butyrate on active sodium and chloride transport in rat and rabbit distal colon"],"prefix":"10.1113","volume":"539","author":[{"given":"S.","family":"Vidyasagar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. S.","family":"Ramakrishna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.031aa.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1016\/S0021-9258(18)53959-4","article-title":"Regulation of the cystic fibrosis transmembrane conductance regulator Cl\u2212 channel by specific protein kinases and protein phosphatases","volume":"268","author":"Berger H. A.","year":"1993","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)90905-X"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017542"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(89)91614-4"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370654"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(87)90433-0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.2.C477"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.274.3.G518"},{"key":"e_1_2_5_11_1","first-page":"1041","article-title":"Pharmacological characterization of stably transfected Na+\/H+ antiporter isoforms using amiloride analogs and a new inhibitor exhibiting anti\u2010ischemic properties","volume":"44","author":"Counillon L.","year":"1993","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.6.C1853"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.278.5.G718"},{"key":"e_1_2_5_14_1","first-page":"G611","article-title":"Mechanism of active potassium absorption and secretion in the rat colon","volume":"246","author":"Foster E. S.","year":"1984","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_15_1","first-page":"G668","article-title":"Corticosteroid alteration of active electrolyte transport in rat distal colon","volume":"245","author":"Foster E. S.","year":"1983","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1993.264.1.E68"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/363263a0"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.1.G259"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.561bt.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.2.G491"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.51.030189.002303"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(86)80021-X"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.251.2.C252"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.276.3.G781"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370587"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1996.270.1.G29"},{"key":"e_1_2_5_27_1","first-page":"G1071","article-title":"Ion transport in rabbit proximal colon: effects of sodium, amiloride, cAMP, and epinephrine","volume":"266","author":"Hyun C. S.","year":"1994","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_28_1","first-page":"G539","article-title":"Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon","volume":"276","author":"Ikuma M.","year":"1999","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018871412748"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.242.1.C81"},{"key":"e_1_2_5_31_1","first-page":"L728","article-title":"Cloning, expression, and tissue distribution of a human amiloride\u2010sensitive Na+ channel","volume":"266","author":"McDonald F. J.","year":"1994","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_32_1","doi-asserted-by":"crossref","first-page":"C693","DOI":"10.1152\/ajpcell.1998.275.3.C693","article-title":"Regulation of apical membrane Na+\/H+ exchangers NHE2 and NHE3 in intestinal epithelial cell line C2\/bbe","volume":"275","author":"McSwine R. L.","year":"1998","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.272.4.G705"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900500"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90008-9"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1042"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.6.C1503"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.4.G687"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00581556"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3360705"},{"key":"e_1_2_5_41_1","first-page":"G874","article-title":"Cl\u2010HCO3 and Cl\u2010OH exchanges mediate Cl uptake in apical membrane vesicles of rat distal colon","volume":"264","author":"Rajendran V. M.","year":"1993","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00232873"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1159\/000200229"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/127.5.838S"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00582529"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374823"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.13.11.915"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00582139"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)90333-7"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(99)00163-7"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)90904-8"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1699"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870702"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00580277"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.4.1466"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.35.24753"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2001.013056","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013056","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2001.013056","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T06:44:21Z","timestamp":1693809861000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2001.013056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2]]},"references-count":55,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,2]]}},"alternative-id":["10.1113\/jphysiol.2001.013056"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2001.013056","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2]]}}}