{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:14:00Z","timestamp":1778598840398,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>In vascular smooth muscle cells many agonists cause the release of Ca<jats:sup>2+<\/jats:sup> ions from internal stores. An important problem concerns the mechanism by which the intracellular stores are refilled subsequent to depletion. In the present study, we describe the properties of a Ca<jats:sup>2+<\/jats:sup>\u2010permeable non\u2010selective cation channel current that is activated in rabbit portal vein myocytes by depletion of internal Ca<jats:sup>2+<\/jats:sup> stores. Application of cyclopiazonic acid (CPA), which depletes internal Ca<jats:sup>2+<\/jats:sup> stores, activated whole\u2010cell currents that had a reversal potential (<jats:italic>E<\/jats:italic><jats:sub>r<\/jats:sub>) of about +50 mV in 1.5 m<jats:bold><jats:sc>m<\/jats:sc><\/jats:bold> external Ca<jats:sup>2+<\/jats:sup> (Ca<jats:bold><jats:sup>2<\/jats:sup><jats:sub>o<\/jats:sub><jats:sup>+<\/jats:sup><\/jats:bold>). In 0 m<jats:sc>m<\/jats:sc> Ca<jats:bold><jats:sup>2<\/jats:sup><jats:sub>o<\/jats:sub><jats:sup>+<\/jats:sup><\/jats:bold>, the currents were larger and <jats:italic>E<\/jats:italic><jats:sub>r<\/jats:sub> was \u223c0 mV. Application of CPA and caffeine during cell\u2010attached recording activated single inward channel currents at negative potentials, which had a slope conductance of 2\u20133 pS and an <jats:italic>E<\/jats:italic><jats:sub>r<\/jats:sub> of +20 mV. The slope conductance in 0 and 110 m<jats:sc>m<\/jats:sc> Ca<jats:bold><jats:sup>2<\/jats:sup><jats:sub>o<\/jats:sub><jats:sup>+<\/jats:sup><\/jats:bold> was 7 and 1.5 pS, respectively, and <jats:italic>E<\/jats:italic><jats:sub>r<\/jats:sub> values indicated that these non\u2010selective cation channels are highly permeable to Ca<jats:sup>2+<\/jats:sup> ions. Bath application of the cell\u2010permeant Ca<jats:sup>2+<\/jats:sup> chelator, BAPTA\u2010AM, also activated similar currents, indicating that these channels are not activated by Ca<jats:sup>2+<\/jats:sup>. Spontaneous channel currents with similar properties to store\u2010operated channels were observed in some patches. Application of W\u20107, an inhibitor of the Ca<jats:sup>2+<\/jats:sup>\u2010binding protein calmodulin, also activated similar Ca<jats:sup>2+<\/jats:sup>\u2010permeable channel currents. In conclusion, it is demonstrated that agents that deplete Ca<jats:sup>2+<\/jats:sup> stores and inhibit calmodulin binding activate Ca<jats:sup>2+<\/jats:sup>\u2010permeable non\u2010selective cation channel currents in rabbit portal vein myocytes. These channels may have an important role in vascular smooth muscle in providing an influx of Ca<jats:sup>2+<\/jats:sup> to refill depleted internal Ca<jats:sup>2+<\/jats:sup> stores and appear to possess different characteristics to store\u2010operated channels described in other vascular smooth muscle preparations.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013101","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"717-728","source":"Crossref","is-referenced-by-count":80,"title":["A Ca<sup>2+<\/sup>\u2010permeable non\u2010selective cation channel activated by depletion of internal Ca<sup>2+<\/sup> stores in single rabbit portal vein myocytes"],"prefix":"10.1113","volume":"538","author":[{"given":"A. P.","family":"Albert","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. A.","family":"Large","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,2]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"80","article-title":"Store\u2010operated inward ion channels in rabbit portal vein myocytes","volume":"531","author":"Albert A. P.","year":"2001","journal-title":"Journal of Physiology"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016333"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3120001"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0609e.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1152\/ajpheart.2001.280.2.H746","article-title":"Upregulated TRP and enhanced capacitative Ca2+ entry in human pulmonary artery myocytes during proliferation. American Journal of Physiology \u2010","volume":"280","author":"Golovina V. A.","year":"2001","journal-title":"Heart and Circulatory Physiology"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.2000.0180"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.053bc.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"e_1_2_5_10_1","first-page":"337","volume-title":"Ionic Channels of Excitable Membranes","author":"Hille B.","year":"1992"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/355353a0"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1152\/ajpcell.2000.278.2.C235","article-title":"Calcium sparks in smooth muscle","volume":"278","author":"Jagger J. H.","year":"2000","journal-title":"American Journal of Physiology \u2014 Cell Physiology"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1152\/ajpcell.1996.271.2.C435","article-title":"Characteristics and physiological role of the calcium\u2010activated chloride conductance in smooth muscle","volume":"271","author":"Large W. A.","year":"1996","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.901"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004666200"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010104200"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00675-8"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019177"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15228.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(99)00400-8"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051632698"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2001.013101","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2001.013101","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T07:42:13Z","timestamp":1693813333000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2001.013101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,2]]}},"alternative-id":["10.1113\/jphysiol.2001.013101"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2001.013101","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2]]}}}