{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T14:39:17Z","timestamp":1770475157880,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p>Inhibition of cAMP\u2010dependent stimulation of vectorial fluid transport across the alveolar epithelium following haemorrhagic shock is mediated by reactive nitrogen species released within the airspaces of the lung. We tested here the hypothesis that the prior activation of the cellular heat shock or stress response, via exposure to either heat or geldanamycin, would attenuate the release of airspace nitric oxide (NO) responsible for the shock\u2010mediated failure of the alveolar epithelium to respond to catecholamines in rats. Rats were haemorrhaged to a mean arterial pressure of 30\u201335 mmHg for 60 min, and then resuscitated with a 4 % albumin solution. Alveolar fluid clearance was measured by change in concentration of a protein solution instilled into the airspaces 5 h after the onset of haemorrhage. Stress preconditioning restored the cAMP\u2010mediated upregulation of alveolar liquid clearance after haemorrhage. The protective effect of stress preconditioning was mediated in part by a decrease in the expression of iNOS in the lung. Specifically, stress preconditioning decreased the production of nitrite by endotoxin\u2010stimulated alveolar macrophages removed from haemorrhaged rats or by A549 and rat alveolar epithelial type II cell monolayers stimulated with cytomix (a mixture of TNF\u2010\u03b1, IL\u20101\u03b2 and IFN\u2010\u03b3) for 24 h. In summary, these results provide the first <jats:italic>in vivo<\/jats:italic> evidence that stress preconditioning restores a normal fluid transport capacity of the alveolar epithelium in the early phase following haemorrhagic shock by attenuating NO\u2010mediated oxidative stress to the lung epithelium.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013102","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"583-597","source":"Crossref","is-referenced-by-count":37,"title":["Stress preconditioning attenuates oxidative injury to the alveolar epithelium of the lung following haemorrhage in rats"],"prefix":"10.1113","volume":"538","author":[{"given":"J. F.","family":"Pittet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. N.","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Geiser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Matthay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"Welch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.4049\/jimmunol.161.5.2594","article-title":"The heat shock response inhibits RANTES gene expression on cultured lung epithelium","volume":"161","author":"Ayad O.","year":"1998","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117989"},{"key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.4049\/jimmunol.157.4.1630","article-title":"In vivo antioxidant treatment suppresses nuclear factor \u03baB activation and neutrophilic lung inflammation","volume":"157","author":"Blackwell T. S.","year":"1996","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"24874","DOI":"10.1016\/S0021-9258(18)40087-7","article-title":"Transcriptional repression of the prointerleukin\u20101\u03b2 gene by heat shock factor 1","volume":"271","author":"Cahil C. M.","year":"1996","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.5.C1417"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.30.17724"},{"key":"e_1_2_5_8_1","first-page":"L236","article-title":"Transforming growth factor\u2010\u03b1 increases alveolar liquid clearance in anesthetized, ventilated rats","volume":"271","author":"Folkesson H. G.","year":"1996","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.274.3.L369"},{"key":"e_1_2_5_10_1","first-page":"L501","article-title":"Pulmonary alveolar epithelial inducible NO synthase gene expression: regulation by inflammatory mediators","volume":"268","author":"Gutierrez H. H.","year":"1995","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041650122"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.6.917"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(99)00164-2"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.151.2.7842182"},{"key":"e_1_2_5_15_1","first-page":"L1098","article-title":"Nitric oxide activates chloride currents in human lung epithelial cells","volume":"272","author":"Kamosinska B.","year":"1997","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_16_1","first-page":"L760","article-title":"\u03b1\u2010Adrenergic blockade restores the normal fluid transport capacity of the alveolar epithelium after hemorrhagic shock","volume":"277","author":"Laffon M.","year":"1999","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041150203"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"3976","DOI":"10.4049\/jimmunol.159.8.3976","article-title":"In vivo inhibition of nuclear factor \u03baB activation prevents inducible nitric oxide synthase expression and systemic hypotension in a rat model of septic shock","volume":"159","author":"Liu S. F.","year":"1997","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/142.6_Pt_1.1250"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117655"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1999.276.5.L844"},{"key":"e_1_2_5_22_1","first-page":"L305","article-title":"Upregulation of alveolar liquid clearance after fluid resuscitation for hemorrhagic shock in rats","volume":"273","author":"Modelska K. M.","year":"1997","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_23_1","volume-title":"Stress Proteins in Biology and Medicine","author":"Morimoto R. I.","year":"1990"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0160-5402(85)90029-4"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.81.2.830"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.10.6301"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117383"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.135.7.860"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.154.6.8970379"},{"key":"e_1_2_5_30_1","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.4049\/jimmunol.141.6.2027","article-title":"Down\u2010regulation of IL\u2010\u03b2 biosynthesis by inducers of the heat shock response","volume":"141","author":"Schmidt J. A.","year":"1988","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_31_1","first-page":"L729","article-title":"Hemorrhage activates NF\u2010\u03baB in murine lung mononuclear cells in vivo","volume":"270","author":"Shenkar R.","year":"1996","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.51.2.181"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.13.1371"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/147.1.177"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199406000-00007"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.6.2004035"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.12.6.7539274"},{"key":"e_1_2_5_38_1","first-page":"L843","article-title":"Expression of inducible nitric oxide synthase in cultured rat pulmonary artery smooth cells is inhibited by the heat shock response","volume":"269","author":"Wong H. R.","year":"1995","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.15.6.8969269"},{"key":"e_1_2_5_40_1","first-page":"L836","article-title":"Increased expression of heat shock protein 70 protects A549 cells against hyperoxia","volume":"275","author":"Wong H. R.","year":"1998","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_41_1","first-page":"L132","article-title":"Selective and transient in vitro effects of heat shock on alveolar type II cell gene expression","volume":"272","author":"Wong H. R.","year":"1997","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199709000-00010"},{"key":"e_1_2_5_43_1","first-page":"1","article-title":"Heat shock activates the I\u2010\u03baBa promoter and increases I\u2010\u03baBa mRNA expression","volume":"4","author":"Wong H. R.","year":"1999","journal-title":"Cell Stress and Chaperones"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6076"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119425"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.1.2005068"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81588-3"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2001.013102","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013102","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2001.013102","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T09:30:27Z","timestamp":1693819827000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2001.013102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1113\/jphysiol.2001.013102"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2001.013102","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1]]}}}