{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:46:14Z","timestamp":1790700374140,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>Proteinase\u2010activated receptor\u20102 (PAR\u20102) may participate in epithelial ion transport regulation. Here we examined the effect of mouse activating peptide (mAP), a specific activator of PAR\u20102, on electrogenic transport of mouse distal colon using short\u2010circuit current (<jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>) measurements. Under steady\u2010state conditions, apical application of amiloride (100 \u03bc<jats:bold><jats:sc>m<\/jats:sc><\/jats:bold>) revealed a positive <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> component of 74.3 \u00b1 6.8 \u03bcA cm<jats:sup>\u22122<\/jats:sup> indicating the presence of Na<jats:sup>+<\/jats:sup> absorption, while apical Ba<jats:sup>2+<\/jats:sup> (10 m<jats:sc>m<\/jats:sc>) identified a negative <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> component of 26.2 \u00b1 1.8 \u03bcA cm<jats:sup>\u22122<\/jats:sup> consistent with K<jats:sup>+<\/jats:sup> secretion. Baseline <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion was minimal. Basolateral addition of 20 \u03bc<jats:sc>m<\/jats:sc> mAP produced a biphasic <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> response with an initial transient peak increase of 11.2 \u00b1 0.9 \u03bcA cm<jats:sup>\u22122<\/jats:sup>, followed by a sustained fall to a level 31.2 \u00b1 2.6 \u03bcA cm<jats:sup>\u22122<\/jats:sup> (<jats:styled-content><jats:italic>n<\/jats:italic>= 43<\/jats:styled-content>) below resting <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub>. The peak response was due to <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion as it was preserved in the presence of amiloride but was largely reduced in the presence of basolateral bumetanide (20 \u03bc<jats:sc>m<\/jats:sc>) or in the absence of extracellular <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>. The secondary decline of <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> was also attenuated by bumetanide and by Ba<jats:sup>2+<\/jats:sup>, indicating that it is partly due to a stimulation of K<jats:sup>+<\/jats:sup> secretion. In addition, the amiloride\u2010sensitive <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> was slightly reduced by mAP, suggesting that inhibition of Na<jats:sup>+<\/jats:sup> absorption also contributes to the <jats:italic>I<\/jats:italic><jats:sub>SC<\/jats:sub> decline. Expression of PAR\u20102 in mouse distal colon was confirmed using RT\u2010PCR and immunocytochemistry. We conclude that functional basolateral PAR\u20102 is present in mouse distal colon and that its activation stimulates <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> and K<jats:sup>+<\/jats:sup> secretion while inhibiting baseline Na<jats:sup>+<\/jats:sup> absorption.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013159","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"209-222","source":"Crossref","is-referenced-by-count":56,"title":["Basolateral PAR\u20102 receptors mediate KCl secretion and inhibition of Na<sup>+<\/sup> absorption in the mouse distal colon"],"prefix":"10.1113","volume":"539","author":[{"given":"John E.","family":"Cuffe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Bertog","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Vel\u00e1zquez\u2010Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Dery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel","family":"Bunnett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Korbmacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00003.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.36.22003"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3141009"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.537bm.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.10.5255"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.5.9912099"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.111.1.127"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0741s.x"},{"key":"e_1_2_5_10_1","first-page":"34P","article-title":"Basolateral proteinase\u2010activated receptor\u20102 modifies transepithelial ion transport in mouse distal colon","volume":"527","author":"Cuffe J. 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