{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:12:54Z","timestamp":1791328374531,"version":"4.3.1"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:p>The cellular mechanisms underlying vasomotion of irideal arterioles from juvenile rats have been studied using electrophysiological methods, ratiometric calcium measurements and video microscopy. Vasomotion was not affected by removal of the endothelium. Spontaneous contractions were preceded by spontaneous depolarizations. Both were abolished by the intracellular calcium chelator, BAPTA AM (20 \u03bc<jats:sc>m<\/jats:sc>), but not by ryanodine (10 \u03bc<jats:sc>m<\/jats:sc>), suggesting a dependence on the cyclical release of calcium from intracellular stores, other than those operated by ryanodine receptors. Oscillations were little changed when the membrane potential of short segments of arteriole was either depolarized or hyperpolarized. When the segments were voltage clamped, oscillating inward currents were recorded, indicating that the changes in membrane potential were voltage independent. Vasomotion was preceded by intracellular calcium oscillations and both were abolished by inhibitors of phospholipase C (U73122, 10 \u03bc<jats:sc>m<\/jats:sc>), phospholipase A<jats:sub>2<\/jats:sub> (AACOCF<jats:sub>3<\/jats:sub>, 30 \u03bc<jats:sc>m<\/jats:sc>) and protein kinase C (chelerythrine chloride, 5 \u03bc<jats:sc>m<\/jats:sc>, and myristoylated protein kinase C peptide, 10 \u03bc<jats:sc>m<\/jats:sc>). Inhibition of vasomotion by the dual lipoxygenase and cyclo\u2010oxygenase inhibitor, NDGA (10 \u03bc<jats:sc>m<\/jats:sc>), the lipoxygenase inhibitor, ETI (1 \u03bc<jats:sc>m<\/jats:sc>) but not by the cyclo\u2010oxygenase inhibitors, aspirin (10 \u03bc<jats:sc>m<\/jats:sc>) and indomethacin (10 \u03bc<jats:sc>m<\/jats:sc>), or the cytochrome P450 inhibitor 17\u2010ODYA (10 \u03bc<jats:sc>m<\/jats:sc>), suggested an involvement of the lipoxygenase pathway. The observations suggest that vasomotion of iris arterioles is voltage independent and results from the cyclical release of calcium from IP<jats:sub>3<\/jats:sub>\u2010sensitive stores which are activated by cross talk between the phospholipase C and phospholipase A<jats:sub>2<\/jats:sub> pathways in vascular smooth muscle.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013698","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"219-229","source":"Crossref","is-referenced-by-count":59,"title":["Voltage independence of vasomotion in isolated irideal arterioles of the rat"],"prefix":"10.1113","volume":"540","author":[{"given":"R. E.","family":"Haddock","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. D. S.","family":"Hirst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. E.","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,4]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000115278"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(00)00452-0"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.2.H475"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.1994.1039"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410x.2001.02135.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01966461"},{"key":"e_1_2_5_8_1","first-page":"H478","article-title":"Spontaneous vasomotion in hamster cheek pouch arterioles in varying experimental conditions","volume":"262","author":"Bouskela E.","year":"1992","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.23679"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1984.0067"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.4.484"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000158942"},{"key":"e_1_2_5_13_1","first-page":"283","article-title":"Prostanoid and leukotriene receptors: a progress report from the IUPHAR working parties on classification and nomenclature","volume":"23","author":"Coleman R. A.","year":"1995","journal-title":"Advances in Prostaglandin Thromboxane Leukotriene Research"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2001.281.1.R246"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018297"},{"key":"e_1_2_5_16_1","first-page":"H1829","article-title":"Vasomotion of basilar arteries in vivo","volume":"258","author":"Fujii K.","year":"1990","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/6.3.315"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(94)90004-3"},{"key":"e_1_2_5_19_1","first-page":"H2469","article-title":"Alpha\u2010adrenoceptor activation of a chloride conductance in rat iris arterioles","volume":"271","author":"Gould D. J.","year":"1996","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.85.6.2255"},{"key":"e_1_2_5_21_1","first-page":"1","article-title":"Vasomotion and underlying mechanisms in small arteries. An in vitro study of rat blood vessels","volume":"614","author":"Gustafsson H.","year":"1993","journal-title":"Acta Physiologica Scandanavica"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1993.tb09623.x"},{"key":"e_1_2_5_23_1","first-page":"77P","article-title":"Involvement of multiple signalling pathways in spontaneous rythmic contractions of rat iris arterioles","volume":"30","author":"Haddock R. E.","year":"1999","journal-title":"Proceedings of the Australian Physiological and Pharmacological Society"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.81.5.812"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.459ae.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.2170\/jjphysiol.36.783"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00507.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.797ba.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.2.546"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.273.5.H2465"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1980.sp013269"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4863-8_66"},{"key":"e_1_2_5_33_1","first-page":"H67","article-title":"Vasomotion in cerebral microcirculation of awake rabbits","volume":"254","author":"Hundley W. G.","year":"1988","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(94)90132-5"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.73.4.751"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.2001.0219"},{"key":"e_1_2_5_37_1","first-page":"800","article-title":"A second cysteinyl leukotriene receptor in human lung","volume":"263","author":"Labat C.","year":"1992","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_5_38_1","doi-asserted-by":"crossref","first-page":"C1129","DOI":"10.1152\/ajpcell.1998.274.4.C1129","article-title":"Norepinephrine stimulates arachidonic acid release from vascular smooth muscle via activation of cPLA2","volume":"274","author":"LaBelle E. F.","year":"1998","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374771"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5458.1647"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021780"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1993.86"},{"key":"e_1_2_5_43_1","first-page":"803","article-title":"Leukotriene D4 receptor\u2010mediated phosphoinositol hydrolysis and calcium mobilization in rat basophilic leukemic cells","volume":"247","author":"Mong S.","year":"1988","journal-title":"Journal of Pharmacology and Experimental Therapeutics."},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.7.596"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1992.tb09444.x"},{"key":"e_1_2_5_46_1","first-page":"48","article-title":"Measurement of arteriole diameter changes by analysis of television images","volume":"26","author":"Neild T. O.","year":"1989","journal-title":"Blood Vessels"},{"key":"e_1_2_5_47_1","first-page":"159","article-title":"Correlation between leukotriene D4\u2010induced contraction and cytosolic calcium elevation: a quantitative and simultaneous evaluation in smooth muscle","volume":"268","author":"Oliva D.","year":"1994","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.2170\/jjphysiol.43.511"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1995.tb09893.x"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(95)02042-X"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704270"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199901)21:1<38::AID-BIES5>3.0.CO;2-S"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.2.H550"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(90)90907-4"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.263.1.H1"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.1999.03030.x"},{"key":"e_1_2_5_57_1","doi-asserted-by":"crossref","first-page":"20976","DOI":"10.1016\/S0021-9258(17)45313-0","article-title":"Leukotriene D4 and E4 induce transmembrane signaling in human epithelial cells. Single cell analysis reveals diverse pathways at the G\u2010protein level for the influx and the intracellular mobilization of Ca2+","volume":"265","author":"Sjolander A.","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/372231a0"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019910"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00245.x"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019888"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021404"},{"key":"e_1_2_5_63_1","first-page":"567","article-title":"Cysteinyl\u2010leukotriene receptors in pulmonary vessels","volume":"50","author":"Walch L.","year":"1999","journal-title":"Journal of Physiology and Pharmacology"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2001.013698","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013698","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2001.013698","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2001.013698","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T23:19:35Z","timestamp":1693869575000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2001.013698"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1113\/jphysiol.2001.013698"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2001.013698","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]}}}