{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T16:12:22Z","timestamp":1789315942396,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:p>Interstitial cells of Cajal (ICC) provide pacemaker activity in some smooth muscles. The nature of the pacemaker conductance is unclear, but studies suggest that pacemaker activity is due to a voltage\u2010independent, Ca<jats:sup>2+<\/jats:sup>\u2010regulated, non\u2010selective cation conductance. We investigated Ca<jats:sup>2+<\/jats:sup>\u2010regulated conductances in murine intestinal ICC and found that reducing cytoplasmic Ca<jats:sup>2+<\/jats:sup> activates whole\u2010cell inward currents and single\u2010channel currents. Both the whole\u2010cell currents and single\u2010channel currents reversed at 0 mV when the equilibrium potentials of all ions present were far from 0 mV. Recordings from on\u2010cell patches revealed oscillations in unitary currents at the frequency of pacemaker currents in ICC. Voltage\u2010clamping cells to \u221260 mV did not change the oscillatory activity of channels in on\u2010cell patches. Depolarizing cells with high external K<jats:sup>+<\/jats:sup> caused loss of resolvable single\u2010channel currents, but the oscillatory single\u2010channel currents were restored when the patches were stepped to negative potentials. Unitary currents were also resolved in excised patches. The single\u2010channel conductance was 13 pS, and currents reversed at 0 mV. The channels responsible were strongly activated by 10<jats:sup>\u22127<\/jats:sup><jats:sc>m<\/jats:sc> Ca<jats:sup>2+<\/jats:sup>, and 10<jats:sup>\u22126<\/jats:sup><jats:sc>m<\/jats:sc> Ca<jats:sup>2+<\/jats:sup> reduced activity. The 13 pS channels were strongly activated by the calmodulin inhibitors calmidazolium and W\u20107 in on\u2010cell and excised patches. Calmidazolium and W\u20107 also activated a persistent inward current under whole\u2010cell conditions. Murine ICC express Ca<jats:sup>2+<\/jats:sup>\u2010inhibited, non\u2010selective cation channels that are periodically activated at the same frequency as pacemaker currents. This conductance may contribute to the pacemaker current and generation of electrical slow waves in GI muscles.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.014639","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"803-814","source":"Crossref","is-referenced-by-count":114,"title":["A Ca<sup>2+<\/sup>\u2010inhibited non\u2010selective cation conductance contributes to pacemaker currents in mouse interstitial cell of Cajal"],"prefix":"10.1113","volume":"540","author":[{"given":"Sang Don","family":"Koh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Yeoul","family":"Jun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Wan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenton M.","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02253840"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.78.5.936"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.515ae.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.2.C529"},{"key":"e_1_2_5_6_1","first-page":"157","article-title":"Sull'ultrastruttura della tunica muscolare della prozione cardiale dell'esofago e dello stomaco umano con particolare riferimento alle cosiddette cellule inerstiziali di Cajal","volume":"82","author":"Faussone\u2010Pellegrini M. 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