{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:06:42Z","timestamp":1792570002105,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p>High tensions of carbon monoxide (CO), relative to oxygen, were used as a tool to investigate the mechanism of chemotransduction. In an <jats:italic>in vitro<\/jats:italic> whole organ, rat carotid body preparation, CO increased sinus nerve chemoafferent discharge in the dark, an effect that was significantly reduced (by <jats:italic>ca<\/jats:italic> 70 %) by bright white light and by the removal of extracellular Ca<jats:sup>2+<\/jats:sup> from the superfusate or by the addition of either Ni<jats:sup>2+<\/jats:sup> (2 mM) or methoxyverapamil (100 \u03bcM). Addition of the P<jats:sub>2<\/jats:sub> purinoceptor antagonist pyridoxalphosphate\u20106\u2010azophenyl\u20102\u2032,4\u2032\u2010disulphonic acid (50 \u03bcM) also significantly reduced the neural response to CO. In perforated patch, whole\u2010cell recordings of isolated rat type I cells, CO induced a depolarisation of <jats:italic>ca<\/jats:italic> 11 mV and a decrease in the amplitude of an outward current around and above the resting membrane potential. Membrane conductance between \u201050 and \u201060 mV was significantly reduced by <jats:italic>ca<\/jats:italic> 40 % by CO. These effects were not photolabile and were present also when a \u2018blocking solution\u2019 containing TEA, 4\u2010AP, Ni<jats:sup>2+<\/jats:sup> and zero extracellular Ca<jats:sup>2+<\/jats:sup> was used. In conventional whole\u2010cell recordings, CO only decreased current amplitudes above +10 mV and was without effect around the resting membrane potential. These data demonstrate a direct effect of CO upon type I cell K<jats:sup>+<\/jats:sup> conductances and strongly suggest an effect upon a background, leak conductance that requires an intracellular mediator. The photolabile effect of CO only upon afferent neural discharge adds further evidence to a dual site of action of CO with a separate action at the afferent nerve terminal that, additionally, requires the permissive action of the neurotransmitter ATP.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.015750","type":"journal-article","created":{"date-parts":[[2002,9,13]],"date-time":"2002-09-13T17:21:43Z","timestamp":1031937703000},"page":"933-945","source":"Crossref","is-referenced-by-count":25,"title":["A Possible Dual Site of Action for Carbon Monoxide\u2010Mediated Chemoexcitation in the Rat Carotid Body"],"prefix":"10.1113","volume":"543","author":[{"given":"C.","family":"Barb\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Al\u2010Hashem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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