{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:55:30Z","timestamp":1791521730792,"version":"4.3.4"},"reference-count":73,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:p>The whole\u2010cell configuration of the patch\u2010clamp technique, immunoprecipitation experiments and unilateral naris occlusions were used to investigate whether the voltage\u2010gated potassium channel Kv1.3 was a substrate for neurotrophin\u2010induced tyrosine phosphorylation and subsequent functional modulation of current properties in cultured rat olfactory bulb (OB) neurons. Membrane proteins of the OB included all three Trk receptor kinases, but the truncated form of the receptor, lacking an intact kinase domain, was the predominant form of the protein for TrkA and TrkC, while TrkB was predominantly found as the full\u2010length receptor. Acute (15 min) stimulation of OB neurons with bath application of 50 ng ml<jats:sup>\u22121<\/jats:sup> brain\u2010derived neurotrophic factor (BDNF), which is a selective ligand for TrkB, caused suppression of the whole\u2010cell outward current and no changes in the kinetics of inactivation or deactivation. Acute stimulation with either nerve growth factor or neurotrophin\u20103 failed to evoke any changes in Kv1.3 function in the OB neurons. Chronic exposure to BDNF (days) caused an increase in the magnitude of Kv1.3 current and speeding of the inactivation and deactivation of the channel. Acute BDNF\u2010induced activation of TrkB receptors significantly increased tyrosine phosphorylation of Kv1.3 in the OB, as shown using a combined immunoprecipitation and Western blot analysis. With unilateral naris occlusion, the acute BDNF\u2010induced tyrosine phosphorylation of Kv1.3 was increased in neurons lacking odour sensory experience. In summary, the duration of neurotrophin exposure and the sensory\u2010dependent state of a neuron can influence the degree of phosphorylation of a voltage\u2010gated ion channel and its concomitant functional modulation by neurotrophins.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.017376","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:15:15Z","timestamp":1027898115000},"page":"413-429","source":"Crossref","is-referenced-by-count":89,"title":["Neurotrophin modulation of voltage\u2010gated potassium channels in rat through TrkB receptors is time and sensory experience dependent"],"prefix":"10.1113","volume":"542","author":[{"given":"K.","family":"Tucker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"Fadool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90507-X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1561"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-19-07417.2000"},{"key":"e_1_2_5_5_1","first-page":"257","article-title":"Neurotrophins BDNF and NT\u20103 promote axonal re\u2010entry into the distal host spinal cord through Schwann cell\u2010seeded mini\u2010channels","volume":"13","author":"Bamber N. I.","year":"2001","journal-title":"European Journal of Neuroscience"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006123-200011000-00006"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(96)80115-2"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/44727"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)64033-9"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-09-03221.2000"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.110.5.601"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005729329526"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","unstructured":"Carter L. A.&Roskams A. J.(2002).Neurotrophins and their receptors in the primary olfactory neuroaxis. (In the Press).","DOI":"10.1002\/jemt.10148"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.20.9444"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-10-03123.1996"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1997.78.3.1563"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-16-06126.1998"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.83.4.2332"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00080-5"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.2001.03417.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903130107"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5295.2089"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199907130-00037"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90324-J"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90405-0"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/79388"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)02285-4"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90234-Q"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/371119a0"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/44847"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00092-1"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1996.66010330.x"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(98)00040-6"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7480"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1992.tb00155.x"},{"key":"e_1_2_5_36_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1523\/JNEUROSCI.15-01-00253.1995","article-title":"Regulation of voltage\u2010gated ion channels by NGF and ciliary neurotrophic factor in SK\u2010 SH neuroblastoma cells","volume":"15","author":"Lesser S. S.","year":"1995","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1997.0656"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/376737a0"},{"key":"e_1_2_5_39_1","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1523\/JNEUROSCI.15-04-03084.1995","article-title":"Differential effects of NGF and BDNF on voltage\u2010gated calcium currents in embryonic basal forebrain neurons","volume":"15","author":"Levine E. S.","year":"1995","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7079"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)03235-2"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(01)00144-4"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00009-5"},{"key":"e_1_2_5_44_1","first-page":"81","article-title":"Mechanism and modulation of inactivation of the Kv3 potassium channel","volume":"1","author":"Marom S.","year":"1993","journal-title":"Receptors and Channels"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490350503"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90243-2"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90292-A"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(99)00655-2"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2000.17.1219"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0240"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2000.00978.x"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20001225)428:4<671::AID-CNE7>3.0.CO;2-H"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1995.tb01079.x"},{"key":"e_1_2_5_54_1","doi-asserted-by":"crossref","first-page":"9596","DOI":"10.1523\/JNEUROSCI.17-24-09596.1997","article-title":"Experience\u2010dependent modifications in MAP2 phosphorylation in rat olfactory bulb","volume":"17","author":"Philpot B.","year":"1997","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/35049004"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/35072576"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-10-03803.1998"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000183200"},{"key":"e_1_2_5_59_1","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1523\/JNEUROSCI.16-04-01294.1996","article-title":"Sequential expression of Trks A, B, and C in the regenerating olfactory neuroepithelium","volume":"16","author":"Roskams A. J. I.","year":"1996","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80563-2"},{"key":"e_1_2_5_61_1","volume-title":"Molecular Cloning, A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90177-F"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.11.5917"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-16-07025.1999"},{"key":"e_1_2_5_65_1","first-page":"219","article-title":"Neurotrophin infusion improves cognitive deficits and decreases cholinergic neuronal cell loss after experimental brain injury","volume":"43","author":"Sinson G.","year":"1996","journal-title":"Clinical Neurosurgery"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/373536a0"},{"key":"e_1_2_5_67_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-24-09492.1997"},{"key":"e_1_2_5_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(99)00552-7"},{"key":"e_1_2_5_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/369233a0"},{"key":"e_1_2_5_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(96)00613-6"},{"key":"e_1_2_5_71_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5300.674"},{"key":"e_1_2_5_72_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7148"},{"key":"e_1_2_5_73_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1998.0684"},{"key":"e_1_2_5_74_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1040971"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2002.017376","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2002.017376","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2002.017376","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2002.017376","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T19:49:43Z","timestamp":1693856983000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2002.017376"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,7]]},"references-count":73,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,7]]}},"alternative-id":["10.1113\/jphysiol.2002.017376"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2002.017376","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,7]]}}}