{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:57:25Z","timestamp":1773291445730,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:p>Rabbit carotid body (CB) chemoreceptor cells possess a fast\u2010inactivating K<jats:sup>+<\/jats:sup> current that is specifically inhibited by hypoxia. We have studied the expression of Kv\u03b1 subunits, which might be responsible for this current. RT\u2010PCR experiments identified the expression of Kv1.4, Kv3.4, Kv4.1 and Kv4.3 mRNAs in the rabbit CB. There was no expression of Kv3.3 or Kv4.2 transcripts. Immunocytochemistry with antibodies to tyrosine hydroxylase (anti\u2010TH) and to specific Kv subunits revealed the expression of Kv3.4 and Kv4.3 in chemoreceptor cells, while Kv1.4 was only found in nerve fibres. Kv4.1 mRNA was also found in chemoreceptor cells following <jats:italic>in situ<\/jats:italic> hybridization combined with anti\u2010TH antibody labelling. Kv4.1 and Kv4.3 appeared to be present in all chemoreceptor cells, but Kv3.4 was only expressed in a population of them. Electrophysiological experiments applying specific toxins or antibodies demonstrated that both Kv3.4 and Kv4.3 participate in the oxygen\u2010sensitive K<jats:sup>+<\/jats:sup> current of chemoreceptor cells. However, toxin application experiments confirmed a larger contribution of members of the Kv4 subfamily. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> measurements under hypoxic conditions and immunocytochemistry experiments in dispersed CB cells demonstrated the expression of Kv3.4 and Kv4.3 in oxygen\u2010sensitive cells; the presence of Kv3.4 in the chemoreceptor cell membrane was not required for the response to low <jats:italic>P<\/jats:italic><jats:sub>O2<\/jats:sub>. In summary, three Kv subunits (Kv3.4, Kv4.1 and Kv4.3) may be involved in the fast\u2010inactivating outward K<jats:sup>+<\/jats:sup> current of rabbit CB chemoreceptor cells. The homogeneous distribution of the Kv4 subunits in chemoreceptor cells, along with their electrophysiological properties, suggest that Kv4.1, Kv4.3, or their heteromultimers, are the molecular correlate of the oxygen\u2010sensitive K<jats:sup>+<\/jats:sup> channel.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.018382","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:15:15Z","timestamp":1027898115000},"page":"369-382","source":"Crossref","is-referenced-by-count":72,"title":["Molecular identification of Kv\u03b1 subunits that contribute to the oxygen\u2010sensitive K<sup>+<\/sup> current of chemoreceptor cells of the rabbit carotid body"],"prefix":"10.1113","volume":"542","author":[{"given":"Diego","family":"Sanchez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose R.","family":"L\u00f3pez\u2010L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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