{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T12:24:00Z","timestamp":1776947040412,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,8,1]],"date-time":"2002-08-01T00:00:00Z","timestamp":1028160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:p>In small segments of circular smooth muscle bundle isolated from the guinea\u2010pig gastric antrum, depolarization of the tissue with intracellular current stimuli evoked regenerative slow potentials after a refractory period of 5\u201310 s. The refractory period changed inversely with the amplitude and duration of the stimulating depolarization. Thapsigargin (an inhibitor of calcium\u2010ATPase at internal stores), 2\u2010aminoethoxydiphenyl borate (2\u2010APB, an inhibitor of inositol 1,4,5\u2010trisphosphate (IP<jats:sub>3<\/jats:sub>)\u2010receptor\u2010mediated Ca<jats:sup>2+<\/jats:sup> release), and carbonyl cyanide <jats:italic>m<\/jats:italic>\u2010chlorophenyl\u2010hydrazone (a mitochondrial protonophore) reduced the amplitude of slow potentials, with no significant alteration of the refractory period. Bisindolylmaleimide I or chelerythrine (inhibitors of protein kinase C, PKC) increased the refractory period and inhibited the amplitude of slow potentials. These results indicate that the refractory period and amplitude of slow potentials are related to the activation of PKC and the amount of Ca<jats:sup>2+<\/jats:sup> released from the internal stores through activation of IP<jats:sub>3<\/jats:sub> receptors, respectively. Acetylcholine (ACh) reduced the refractory period and increased the amplitude of slow potentials: the former was antagonized by chelerythrine and the latter by 2\u2010APB. The results suggest that ACh has dual actions; stimulation of the metabolism of inositol phosphate and activation of PKC. Phorbol\u201012\u2010myristate\u201013\u2010acetate, a selective stimulant of PKC, at low concentrations (&lt; 10 n<jats:sc>m<\/jats:sc>) mimicked the actions of ACh and at high concentrations reduced the frequency of slow potentials and increased the refractory period. The possible involvement of the concentration\u2010dependent differences in the actions of phorbol ester on the translocation of PKC was considered.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.020875","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T19:28:19Z","timestamp":1033500499000},"page":"155-167","source":"Crossref","is-referenced-by-count":34,"title":["Excitation of smooth muscles isolated from the guinea\u2010pig gastric antrum in response to depolarization"],"prefix":"10.1113","volume":"543","author":[{"given":"Yoshihiko","family":"Kito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyasu","family":"Fukuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimichi","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikaru","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011571200"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050902"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0827h.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.515ae.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.001aa.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0235o.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(85)90347-5"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013306"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019845"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91544-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.014803"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00165.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019681"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/S0165-6147(97)90668-4","article-title":"Interstitial cells of Cajal as targets for pharmacological intervention in gastrointestinal motor disorders","volume":"8","author":"Huizinga J. 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