{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:28:16Z","timestamp":1762144096673},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>To determine the predominant nicotinic ACh receptor (nAChR) located on neurones in the dorsal motor nucleus of the vagus (DMV) that project to the gastrointestinal tract, we used the rat brainstem slice preparation and whole\u2010cell recordings of DMV neurones identified by retrograde DiI tracing to pharmacologically characterize nAChRs. Pressure ejection of acetylcholine (ACh, 250 \u03bc<jats:sc>m<\/jats:sc> for 200 ms) from a patch pipette placed \u224810\u201020 \u03bcm from the surface of the recorded cell produced an inward current in most DMV neurones sampled. The average currents for neurones projecting to the fundus, antrum and caecum were 149 \u00b1 38 (<jats:styled-content><jats:italic>n<\/jats:italic>= 25<\/jats:styled-content>), 115 \u00b1 18 (<jats:styled-content><jats:italic>n<\/jats:italic>= 29<\/jats:styled-content>) and 117 \u00b1 23 pA (<jats:styled-content><jats:italic>n<\/jats:italic>= 6<\/jats:styled-content>), respectively. Blockade of the \u03b17 subtype of nAChR with either \u03b1\u2010bungarotoxin (\u03b1\u2010BGT) or methyllycaconitine (MLA) counteracted 60\u201075 % of the ACh\u2010evoked current in DMV neurones projecting to the fundus, antrum and caecum. In neurones projecting to the fundus and the antrum, currents resistant to \u03b1\u2010BGT were significantly blocked by dihydro\u2010\u03b2\u2010erythroidine (10\u201020 n<jats:sc>m<\/jats:sc>), an antagonist of the \u03b14\u03b22 subtype of nAChR. In neurones projecting to the caecum, currents resistant to \u03b1\u2010BGT were significantly depressed by a low concentration of mecamylamine (1 \u03bc<jats:sc>m<\/jats:sc>). Cytisine (100 \u03bc<jats:sc>m<\/jats:sc>), an agonist of nAChRs that contain the \u03b17 or the \u03b24 subunit, evoked significant currents in caecum\u2010projecting neurones that were previously exposed to \u03b1\u2010BGT. In contrast, cytisine had no effect on DMV neurones previously exposed to \u03b1\u2010BGT that project to the fundus or antrum. Our data indicate that the prevailing nAChR subtype in DMV neurones projecting to the GI tract is the \u03b17 subtype. In addition, we obtained evidence for the co\u2010expression of the \u03b14\u03b22 nAChR subtype on DMV neurones projecting to the fundus and antrum, and the \u03b13\u03b24 nAChR subtype on DMV neurones projecting to the caecum.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.021337","type":"journal-article","created":{"date-parts":[[2002,12,15]],"date-time":"2002-12-15T03:29:24Z","timestamp":1039922964000},"page":"1007-1016","source":"Crossref","is-referenced-by-count":13,"title":["Nicotinic ACH receptor subtypes on gastrointestinally projecting neurones in the dorsal motor vagal nucleus of the rat"],"prefix":"10.1113","volume":"545","author":[{"given":"Niaz","family":"Sahibzada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Manuel","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernice","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Wasserman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Vicini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Gillis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"1455","article-title":"Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. 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