{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T05:45:21Z","timestamp":1792215921584,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>Loading slices of rat barrel cortex with 50 \u03bc<jats:sc>m<\/jats:sc> BAPTA\u2010AM while recording from pyramidal cells in layer II induces a marked reduction in both the frequency and amplitudes of mEPSCs. These changes are due to a presynaptic action. Blocking the refilling of Ca<jats:sup>2+<\/jats:sup> stores with 20 \u03bc<jats:sc>m<\/jats:sc> cyclopiazonic acid (CPA), a SERCA pump inhibitor, in conjunction with neuronal depolarisation to activate Ca<jats:sup>2+<\/jats:sup> stores, results in a similar reduction of mEPSCs to that observed with BAPTA\u2010AM, indicating that the source for intracellular Ca<jats:sup>2+<\/jats:sup> is the endoplasmic reticulum. Block or activation of ryanodine receptors by 20 \u03bc<jats:sc>m<\/jats:sc> ryanodine or 10 m<jats:sc>m<\/jats:sc> caffeine, respectively, shows that a significant proportion of mEPSCs are caused by Ca<jats:sup>2+<\/jats:sup> release from ryanodine stores. Blocking IP<jats:sub>3<\/jats:sub> receptors with 14 \u03bc<jats:sc>m<\/jats:sc> 2\u2010aminoethoxydiphenylborane (2APB) also reduces the frequency and amplitude of mEPSCs, indicating the involvement of IP<jats:sub>3<\/jats:sub> stores in the generation of mEPSCs. Activation of group I metabotropic receptors with 20 \u03bc<jats:sc>m<\/jats:sc> (<jats:italic>RS)<\/jats:italic>\u20103,5\u2010dihydroxyphenylglycine (DHPG) results in a significant increase in the frequency of mEPSCs, further supporting the role of IP<jats:sub>3<\/jats:sub> receptors and indicating a role of group I metabotropic receptors in causing transmitter release. Statistical evidence is presented for Ca<jats:sup>2+<\/jats:sup>\u2010induced Ca<jats:sup>2+<\/jats:sup> release (CICR) from ryanodine stores after the spontaneous opening of IP<jats:sub>3<\/jats:sub> stores.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.022103","type":"journal-article","created":{"date-parts":[[2002,11,28]],"date-time":"2002-11-28T03:06:17Z","timestamp":1038452777000},"page":"521-535","source":"Crossref","is-referenced-by-count":110,"title":["The contribution of intracellular calcium stores to mEPSCs recorded in layer II neurones of rat barrel cortex"],"prefix":"10.1113","volume":"545","author":[{"given":"Christopher R. 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