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Tetraethylammonium (TEA, 4 m<jats:sc>m<\/jats:sc>) application isolated an A\u2010type K<jats:sup>+<\/jats:sup>current (<jats:italic>I<\/jats:italic><jats:sub><jats:sc>k<\/jats:sc>(<jats:sc>a<\/jats:sc>)<\/jats:sub>) with a peak amplitude, at +20 mV, of about one third of the total voltage\u2010dependent and calcium\u2010independent K<jats:sup>+<\/jats:sup>current. The<jats:italic>I<\/jats:italic><jats:sub><jats:sc>k<\/jats:sc>(<jats:sc>a<\/jats:sc>)<\/jats:sub>activated at about \u221260 mV, had a<jats:italic>V<\/jats:italic><jats:sub>0.5<\/jats:sub>of activation of \u221224.9 mV and a<jats:italic>V<\/jats:italic><jats:sub>0.5<\/jats:sub>of inactivation of \u221269.2 mV. The deactivation time constant at \u221270 mV was 3.4 \u00b1 0.4 ms, while the activation time constant at +20 mV was 0.9 \u00b1 0.2 ms. The inactivation kinetics was weakly voltage dependent, with two time constants; those at +20 mV were 19.3 \u00b1 3.1 and 97.6 \u00b1 9.8 ms. The recovery from inactivation had two time constants of 60.8 ms (78.4%) and 962.3 ms (21.6%). The<jats:italic>I<\/jats:italic><jats:sub><jats:sc>k<\/jats:sc>(<jats:sc>a<\/jats:sc>)<\/jats:sub>was blocked by 4\u2010aminopyridine with an IC<jats:sub>50<\/jats:sub>of 67.6 \u03bcM. Agitoxin\u20102 (2 n<jats:sc>m<\/jats:sc>) blocked 17.4 \u00b1 2.1% of the<jats:italic>I<\/jats:italic><jats:sub><jats:sc>k<\/jats:sc>(<jats:sc>a<\/jats:sc>)<\/jats:sub>. Flecainide completely blocked the<jats:italic>I<\/jats:italic><jats:sub><jats:sc>k<\/jats:sc>(<jats:sc>a<\/jats:sc>)<\/jats:sub>with a biphasic effect with IC<jats:sub>50<\/jats:sub>values of 4.4 and 183.2 \u03bcM. In current\u2010clamp recordings the duration of evoked action potentials was affected neither by agitoxin\u20102 (2 n<jats:sc>m<\/jats:sc>) nor by flecainide (3 \u03bcM), but action potentials that were already broadened by TEA were further prolonged by 4\u2010aminopyridine (100 \u03bcM). The amplitude of the hyperpolarisation at the end of depolarising steps was reduced by all these blockers.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.022525","type":"journal-article","created":{"date-parts":[[2002,8,30]],"date-time":"2002-08-30T21:23:00Z","timestamp":1030742580000},"page":"505-520","source":"Crossref","is-referenced-by-count":47,"title":["A\u2010Type potassium currents active at subthreshold potentials in mouse cerebellar purkinje cells"],"prefix":"10.1113","volume":"543","author":[{"given":"Tiziana","family":"Sacco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Tempia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,9]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000592"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.66.3.789"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80761-8"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.5.560"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013127"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015542"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(19980205)34:2<135::AID-NEU4>3.0.CO;2-3"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02068-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb11293.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00236588"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01304-6"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.4.659"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00580998"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1981.sp013859"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002210050516"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017793"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.80.6.3047"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00188a012"},{"key":"e_1_2_5_19_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1523\/JNEUROSCI.14-02-00511.1994","article-title":"Kv3. 3b: a novel Shaw type potassium channel expressed in terminally differentiated cerebellar Purkinje cells and deep cerebellar nuclei","volume":"14","author":"Goldman\u2010Wohl D.S.","year":"1994","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_20_1","first-page":"1227","article-title":"Pharmacological characterization of five cloned voltage\u2010gated K+ channels, types Kv1. 1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines","volume":"45","author":"Grissmer S.","year":"1994","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_5_21_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1523\/JNEUROSCI.11-04-01002.1991","article-title":"Single\u2010channel K+ currents recorded from the somatic and dendritic regions of cerebellar Purkinje neurons in culture","volume":"11","author":"Gruol D. 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