{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:10:50Z","timestamp":1770340250994,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>To determine if pulmonary oedema fluid (EF) alters ion and fluid transport of distal lung epithelium (DLE), EF was collected from rats in acute heart failure. EF, but not plasma, increased amiloride\u2010insensitive short circuit current (<jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub>) and Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup> ATPase protein content and pump activity of DLE grown in primary culture. Inhibitors of <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> transport or cGMP\u2010gated cation channels had a significant (<jats:styled-content><jats:italic>P<\/jats:italic> &lt; 0.05<\/jats:styled-content>), but limited ability to block the increased <jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub>. EF increased amiloride\u2010insensitive, but not amiloride\u2010sensitive, DLE apical membrane Na<jats:sup>+<\/jats:sup> conductance. The level of mRNA encoding epithelial sodium channel (ENaC) subunits was unchanged (\u03b1, \u03b2), or decreased (\u03b3, <jats:styled-content><jats:italic>P<\/jats:italic> &lt; 0.05<\/jats:styled-content>) in EF\u2010exposed DLE. EF also induced an amiloride\u2010insensitive increase in the potential difference across murine tracheal cysts. Distal lung explants from late gestation wild\u2010type and \u03b1\u2010ENaC\u2010deficient fetal mice, which normally expand due to liquid secretion, decreased in size due to liquid absorption when exposed to EF. Trypsin digestion or heat treatment of EF abrogated the ability of EF to increase amiloride\u2010insensitive <jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub> in DLE and liquid absorption by distal lung explants. Thus proteins or protein\u2010dependent factors within cardiogenic EF induce an \u03b1\u2010ENaC\u2010independent and amiloride\u2010insensitive apical membrane Na<jats:sup>+<\/jats:sup> conductance and liquid absorption in the distal lung.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.024612","type":"journal-article","created":{"date-parts":[[2002,10,15]],"date-time":"2002-10-15T16:45:17Z","timestamp":1034700317000},"page":"537-548","source":"Crossref","is-referenced-by-count":38,"title":["Pulmonary oedema fluid induces non\u2010\u03b1\u2010ENaC\u2010dependent Na<sup>+<\/sup> transport and fluid absorption in the distal lung"],"prefix":"10.1113","volume":"544","author":[{"given":"Bijan","family":"Rafii","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Gillie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Sulowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicky","family":"Hannam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gail","family":"Otulakowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre M.","family":"Barker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugh","family":"O'Brodovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0105g.x"},{"key":"e_1_2_5_3_1","first-page":"L512","article-title":"Effect of gas composition on liquid secretion by explants of distal lung of fetal rat in submersion culture","volume":"265","author":"Barker P.","year":"1993","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3971"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.3.9610013"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/361467a0"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/367463a0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0396-248"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.4.C1050"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.5.C1330"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200009000-00007"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.4.C1335"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.274.3.L378"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1995.375"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(96)00114-0"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.25.1.4500"},{"key":"e_1_2_5_17_1","doi-asserted-by":"crossref","first-page":"C21","DOI":"10.1152\/ajpcell.1997.273.1.C21","article-title":"Volume transport across tracheal and bronchial airway epithelia in a tubular culture system","volume":"273","author":"Grubb B. 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