{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:40:04Z","timestamp":1792129204961,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p>We studied granule cells (GCs) in the intact frog olfactory bulb (OB) by combining whole\u2010cell recordings and functional two\u2010photon Ca<jats:sup>2+<\/jats:sup> imaging in an <jats:italic>in vitro<\/jats:italic> nose\u2010brain preparation. GCs are local interneurones that shape OB output via distributed dendrodendritic inhibition of OB projection neurones, the mitral\u2010tufted cells (MTCs). In contrast to MTCs, GCs exhibited a Ca<jats:sup>2+<\/jats:sup>\u2010activated non\u2010specific cation conductance (<jats:italic>I<\/jats:italic><jats:sub>CAN<\/jats:sub>) that could be evoked through strong synaptic stimulation or suprathreshold current injection. Photolysis of the caged Ca<jats:sup>2+<\/jats:sup> chelator <jats:italic>o<\/jats:italic>\u2010nitrophenol\u2010EGTA resulted in activation of an inward current with a reversal potential within the range \u201020 to +10 mV. <jats:italic>I<\/jats:italic><jats:sub>CAN<\/jats:sub> in GCs was suppressed by the intracellular Ca<jats:sup>2+<\/jats:sup> chelator BAPTA (0.5\u20135.0 mM), but not by EGTA (up to 5 mM). The current persisted in whole\u2010cell recordings for up to 1.5 h post\u2010breakthrough, was observed during perforated\u2010patch recordings and was independent of ionotropic glutamate and GABA<jats:sub>A<\/jats:sub> receptor activity. In current\u2010clamp mode, GC responses to synaptic stimulation consisted of an initial AMPA\u2010mediated conductance followed by a late\u2010phase APV\u2010sensitive plateau (100\u2013500 ms). BAPTA\u2010mediated suppression of <jats:italic>I<\/jats:italic><jats:sub>CAN<\/jats:sub> resulted in a selective reduction of the late component of the evoked synaptic potential, consistent with a positive feedback relationship between NMDA receptor (NMDAR) current and <jats:italic>I<\/jats:italic><jats:sub>CAN<\/jats:sub>. <jats:italic>I<\/jats:italic><jats:sub>CAN<\/jats:sub> requires Ca<jats:sup>2+<\/jats:sup> influx either through voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> channels or possibly NMDARs, both of which have a high threshold for activation in GCs, predicting a functional role for this current in the selective enhancement of strong synaptic inputs to GCs.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.024638","type":"journal-article","created":{"date-parts":[[2002,9,13]],"date-time":"2002-09-13T17:21:43Z","timestamp":1031937703000},"page":"819-834","source":"Crossref","is-referenced-by-count":39,"title":["Contribution of a Calcium\u2010Activated Non\u2010Specific Conductance to NMDA Receptor\u2010Mediated Synaptic Potentials in Granule Cells of the Frog Olfactory Bulb"],"prefix":"10.1113","volume":"543","author":[{"given":"Benjamin J.","family":"Hall","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerry R.","family":"Delaney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90913-G"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(99)02342-2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81065-X"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(96)00069-6"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-24-10727.1999"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"8169","DOI":"10.1523\/JNEUROSCI.20-21-08169.2000","article-title":"A cellular mechanism for the transformation of a sensory input into a motor command","volume":"20","author":"Prisco G. V.","year":"2000","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.1.187"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-13-04113.1996"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1972.35.6.762"},{"key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.1523\/JNEUROSCI.20-13-05124.2000","article-title":"Calcium influx through NMDA receptors directly evokes GABA release in olfactory bulb granule cells","volume":"20","author":"Halabisky B.","year":"2000","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(83)90206-3"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1152\/jn.1990.64.6.1838","article-title":"A calcium\u2010dependent slow afterdepolarization recorded in rat dorsolateral septal nucleus neurons in vitro","volume":"64","author":"Hasuo H.","year":"1990","journal-title":"Journal of Neurophysiology"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.70031269.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7361098"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014187"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903200106"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015666"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90496-O"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018331"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)91035-6"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90904-1"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012362"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-17-07309.1999"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(71)90600-7"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1981.46.3.639"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1981.46.3.639"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(94)90017-5"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00581323"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00617.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)02275-7"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.82.2.730"},{"key":"e_1_2_5_33_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1242\/jcs.7.1.125","article-title":"The synaptology of the granule cells of the olfactory bulb","volume":"7","author":"Price J. L.","year":"1970","journal-title":"Journal of Cell Science"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(91)90017-T"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1968.31.6.884"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(66)90023-9"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(93)91427-T"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1997.78.5.2483"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903050307"},{"key":"e_1_2_5_40_1","doi-asserted-by":"crossref","first-page":"6790","DOI":"10.1523\/JNEUROSCI.18-17-06790.1998","article-title":"Dendrodendritic inhibition in the olfactory bulb is driven by NMDA receptors","volume":"18","author":"Schoppa N. E.","year":"1998","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.5.2220"},{"key":"e_1_2_5_42_1","volume-title":"The Synaptic Organization of the Brain","author":"Shepherd G. M.","year":"1998"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199605)30:1<123::AID-NEU11>3.0.CO;2-N"},{"key":"e_1_2_5_44_1","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1152\/jn.1985.54.6.1430","article-title":"Activation of a nonspecific cation conductance by intracellular Ca2+ elevation in bursting pacemaker neurons of Helix pomatia","volume":"54","author":"Swandulla D.","year":"1985","journal-title":"Journal of Neurophysiology"},{"key":"e_1_2_5_45_1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1152\/jn.1990.64.2.598","article-title":"Excitatory synaptic transmission in cultures of rat olfactory bulb","volume":"64","author":"Trombley P. Q.","year":"1990","journal-title":"Journal of Neurophysiology"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020082"},{"key":"e_1_2_5_47_1","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1152\/jn.1990.64.3.932","article-title":"Intracellular responses of identified rat olfactory bulb interneurons to electrical and odor stimulation","volume":"64","author":"Wellis D. P.","year":"1990","journal-title":"Journal of Neurophysiology"},{"key":"e_1_2_5_48_1","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1523\/JNEUROSCI.11-06-01837.1991","article-title":"Local information processing in dendritic trees: subsets of spines in granule cells of the mammalian olfactory bulb","volume":"11","author":"Woolf T. B.","year":"1991","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.8.3371"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2002.024638","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2002.024638","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2002.024638","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2002.024638","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T13:21:30Z","timestamp":1693833690000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2002.024638"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1113\/jphysiol.2002.024638"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2002.024638","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]}}}