{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:28:43Z","timestamp":1759332523222},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>A\u2010type currents are rapidly inactivating potassium currents that operate at subthreshold potentials. A\u2010type currents have not been reported to occur in the phasic muscles of the stomach. We used conventional voltage\u2010clamp techniques to identify and characterize A\u2010type currents in myocytes isolated from the murine antrum. A\u2010type currents were robust in these cells, with peak current densities averaging 30 pA pF<jats:sup>\u22121<\/jats:sup> at 0 mV. These currents underwent rapid inactivation with a time constant of 83 ms at 0 mV. Recovery from inactivation at \u221280 mV was rapid, with a time constant of 252 ms. The A\u2010type current was blocked by 4\u2010aminopyridine (4\u2010AP) and was inhibited by flecainide, with an IC<jats:sub>50<\/jats:sub> of 35 \u03bcM. The voltage for half\u2010activation was \u221226 mV, while the voltage of half\u2010inactivation was \u221265 mV. There was significant activation and incomplete inactivation at potentials positive to \u221260 mV, which is suggestive of sustained current availability in this voltage range. Under current\u2010clamp conditions, exposure to 4\u2010AP or flecainide depolarized the membrane potential by 7\u201010 mV. In intact antral tissue preparations, flecainide depolarized the membrane potential between slow waves by 5 mV; changes in slow waves were not evident. The effect of flecainide was not abolished by inhibiting enteric neurotransmission or by blocking delayed rectifier and ATP\u2010sensitive K<jats:sup>+<\/jats:sup> currents. Transcripts encoding Kv4 channels were detected in isolated antral myocytes by RT\u2010PCR. Immunocytochemistry revealed intense Kv4.2\u2010 and Kv4.3\u2010like immunoreactivity in antral myocytes. These data suggest that the A\u2010type current in murine antral smooth muscle cells is likely to be due to Kv4 channels. This current contributes to the maintenance of negative resting membrane potentials.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.025171","type":"journal-article","created":{"date-parts":[[2002,10,15]],"date-time":"2002-10-15T16:45:17Z","timestamp":1034700317000},"page":"417-428","source":"Crossref","is-referenced-by-count":36,"title":["Characterization of the A\u2010type potassium current in murine gastric antrum"],"prefix":"10.1113","volume":"544","author":[{"given":"Gregory C.","family":"Amberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salah A.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Don","family":"Koh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Hatton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith J.","family":"Murray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burton","family":"Horowitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenton M.","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,10]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"G979","article-title":"Transient outward current in opossum esophageal circular muscle","volume":"268","author":"Akbarali H. I.","year":"1995","journal-title":"American Journal of Physiology"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.025163"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.281.6.C2020"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000592"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.8.5337"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI333"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)90031-0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017623"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1991.sp003535"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009365"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.515ae.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0235o.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.277.1.G127"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.45"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0247r.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.82.1.289"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00220-X"},{"key":"e_1_2_5_19_1","first-page":"1227","article-title":"Pharmacological characterization of five cloned voltage\u2010gated K+ channels, types Kv1. 1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines","volume":"45","author":"Grissmer S.","year":"1994","journal-title":"Molecular Pharmacology"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1961.sp006640"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0237k.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.19"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1152\/jn.1991.66.4.1304","article-title":"A fast transient potassium current in thalamic relay neurons: kinetics of activation and inactivation","volume":"66","author":"Huguenard J. R.","year":"1991","journal-title":"Journal of Neurophysiology"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017565"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.012765"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77621-0"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0075z.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.475ac.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.6.C1523"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.221168498"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019170"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01483-X"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(01)00958-4"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002100000257"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.54.030192.002255"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1994.72.4.1516"},{"key":"e_1_2_5_38_1","first-page":"66","article-title":"K+ channel blocking actions of flecainide compared with those of propafenone and quinidine in adult rat ventricular myocytes","volume":"269","author":"Slawsky M. T.","year":"1994","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019280"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.263.1.C226"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1992.67.3.599"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.3.C527"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.280.5.C1184"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00240-L"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004768200"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00221-0"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704109"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh1001.090839"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.88.5.476"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2002.025171","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2002.025171","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2002.025171","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2002.025171","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T09:58:28Z","timestamp":1693821508000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2002.025171"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,10]]}},"alternative-id":["10.1113\/jphysiol.2002.025171"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2002.025171","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10]]}}}