{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:51:08Z","timestamp":1790423468777,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>A non\u2010genomic antisecretory role for dexamethasone at low concentrations (0.1 n<jats:sc>m<\/jats:sc> to1 \u03bc<jats:sc>m<\/jats:sc>) is described in monolayers of human bronchial epithelial cells in primary culture and in a continuous cell line (16HBE14o\u2010 cells). Dexamethasone produced a rapid decrease of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> (measured with fura\u20102 spectrofluorescence) to a new steady\u2010state concentration. After 15 min exposure to dexamethasone (1 n<jats:sc>m<\/jats:sc>), [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> was reduced by 32 \u00b1 11 n<jats:sc>m<\/jats:sc> (<jats:styled-content><jats:italic>n<\/jats:italic>= 7<\/jats:styled-content>, <jats:styled-content><jats:italic>P<\/jats:italic> &lt; 0.0001<\/jats:styled-content>) from a basal value of 213 \u00b1 36 n<jats:sc>m<\/jats:sc> (<jats:styled-content><jats:italic>n<\/jats:italic>= 7<\/jats:styled-content>). We have shown previously that aldosterone (1 n<jats:sc>m<\/jats:sc>) also produces a rapid fall in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>; however, after the decrease in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> induced by dexamethasone, subsequent addition of aldosterone did not produced any further lowering of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. The rapid response to dexamethasone was insensitive to pretreatment with cycloheximide and unaffected by the glucocorticoid type II and mineralocorticoid receptor antagonists RU486 and spironolactone, respectively. The rapid [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> decrease induced by dexamethasone was inhibited by the Ca<jats:sup>2+<\/jats:sup>\u2010ATPase pump inhibitor thapsigargin (1 \u03bc<jats:sc>m<\/jats:sc>), the adenylate cyclase inhibitor MDL hydrochloride (500 \u03bc<jats:sc>m<\/jats:sc>) and the protein kinase A inhibitor Rp\u2010adenosine 3\u2032,5\u2032\u2010cyclic monophosphorothioate (200 \u03bc<jats:sc>m<\/jats:sc>), but was not affected by the protein kinase C inhibitor, chelerythrine chloride (0.1 \u03bc<jats:sc>m<\/jats:sc>). Treatment of 16HBE14o\u2010 cell monolayers with dexamethasone (1 n<jats:sc>m<\/jats:sc>) inhibited the large and transient [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase induced by apical exposure to ATP (10<jats:sup>\u22124<\/jats:sup><jats:sc>m<\/jats:sc>). Dexamethasone (1 n<jats:sc>m<\/jats:sc>) also reduced by 30 % the Ca<jats:sup>2+<\/jats:sup>\u2010dependant <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion induced by apical exposure to ATP (measured as the <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content>\u2010sensitive short\u2010circuit current across monolayers mounted in Ussing chambers). Our results demonstrate, for the first time, that dexamethasone at low concentrations inhibits <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion in human bronchial epithelial cells. The rapid inhibition of <jats:styled-content>Cl<jats:sup>\u2212<\/jats:sup><\/jats:styled-content> secretion induced by the synthetic glucocorticoid is associated with a rapid decrease in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> via a non\u2010genomic mechanism that does not involve the classical glucocorticoid or mineralocorticoid receptor. Rather, it is a result of rapid non\u2010genomic stimulation of thapsigargin\u2010sensitive Ca<jats:sup>2+<\/jats:sup>\u2010ATPase, via adenylate cyclase and protein kinase A signalling.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.028183","type":"journal-article","created":{"date-parts":[[2002,12,15]],"date-time":"2002-12-15T03:29:24Z","timestamp":1039922964000},"page":"869-878","source":"Crossref","is-referenced-by-count":59,"title":["Rapid non\u2010genomic inhibition of ATP\u2010induced Cl<sup>\u2212<\/sup> secretion by dexamethasone in human bronchial epithelium"],"prefix":"10.1113","volume":"545","author":[{"given":"V.","family":"Urbach","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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