{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:55:03Z","timestamp":1792713303091,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:p>Chronic overexpression of the acetylcholine\u2010hydrolysing enzyme acetylcholinesterase (AChE) is a notable consequence of exposure to anticholinesterase drugs or poisons. However, the physiological consequences for the resultant neuromuscular disfunction have not yet been carefully analysed. Here we report detailed dissection of the different components of neuromuscular function in transgenic mice previously shown to display motor fatigue and altered muscle morphology as a consequence of neuronal overexpression of AChE\u2010S, the synaptic AChE variant. Transgenic diaphragm muscle presented exaggerated fatigue as a combined consequence of neurotransmission fading and muscle mechanical malfunctioning. In a tetanic stimulation protocol, transgenic muscles rapidly fatigued to a larger extent than wild\u2010type muscles, when stimulated either directly or via the phrenic nerve. AChE overexpression involved moderate but significant aberrations of synaptic transmission with higher quantal content (measured at 0.2 m<jats:sc>m<\/jats:sc>Ca<jats:sup>2+<\/jats:sup>, 2.3 m<jats:sc>m<\/jats:sc>Mg<jats:sup>2+<\/jats:sup>). Furthermore, treatment with the anti\u2010cholinesterase physostigmine revealed a higher amplitude and half\u2010decay time of the transgenic quantal postsynaptic response. Our observations imply that elevated levels of neuronal AChE\u2010S are expected to cause muscle exhaustion due to a combination of modest, multilevelled aberrations in synaptic transmission, muscle function and morphology.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.030841","type":"journal-article","created":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T03:57:03Z","timestamp":1041393423000},"page":"165-173","source":"Crossref","is-referenced-by-count":27,"title":["Chronic acetylcholinesterase overexpression induces multilevelled aberrations in mouse neuromuscular physiology"],"prefix":"10.1113","volume":"546","author":[{"given":"Noa","family":"Farchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermona","family":"Soreq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binyamin","family":"Hochner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,1]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1986.61.3.1077"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.15.8173"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-0186(97)00121-6"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.3.755"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90331-X"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00211-9"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69062441.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn788"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90346-1"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mp.4001103"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.1996.0010"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00864.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.59.4.563"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1954.sp005129"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.81.5.2386"},{"key":"e_1_2_5_17_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1093\/oso\/9780195129700.003.0011","volume-title":"Myasthenia 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