{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T08:03:15Z","timestamp":1759564995118},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>Little is known about contractile activity, response to agonists or excitation\u2010contraction coupling in neonatal smooth muscle. We have therefore investigated 10\u2010day rat uterus to better understand these processes, and compared it to adult uterus to elucidate how control of contractility develops. Spontaneous contractions are present in the 10\u2010day neonatal uterus, although they are not as large or as regular as those present in adult tissues. External Ca<jats:sup>2+<\/jats:sup> entry via L\u2010type Ca<jats:sup>2+<\/jats:sup> channels is the sole source of Ca<jats:sup>2+<\/jats:sup> and is essential for the spontaneous activity. The neonatal uterus was responsive to carbachol or prostaglandin F<jats:sub>2\u03b1<\/jats:sub> application; it showed a marked stimulation and a clear dissociation between the force and Ca<jats:sup>2+<\/jats:sup> changes. Such sensitization was not apparent in adult rat myometrium. The sarcoplasmic reticulum (SR) had more releasable Ca<jats:sup>2+<\/jats:sup> and contributed more to the response to agonists in neonatal compared to adult tissues. Thus, Ca<jats:sup>2+<\/jats:sup> entry as opposed to SR Ca<jats:sup>2+<\/jats:sup> release contributed much less to the uterine response to agonists in the neonatal, compared to adult tissues. Inhibition of the SR by cyclopiazonic acid also caused a more vigorous increase in Ca<jats:sup>2+<\/jats:sup> and contractile activity, particularly frequency, in the neonatal compared to the adult uterus. Taken together these data suggest that: (1) spontaneous activity is already present by day 10, (2) receptor\u2010coupling and excitation\u2010contraction signalling pathways are functional, (3) the SR and Ca<jats:sup>2+<\/jats:sup> sensitization mechanisms play a more prominent role in the neonate, and (4) there is a shift to a greater reliance on Ca<jats:sup>2+<\/jats:sup> entry and excitability with development of the myometrium.<\/jats:p>","DOI":"10.1113\/jphysiol.2002.031732","type":"journal-article","created":{"date-parts":[[2002,11,28]],"date-time":"2002-11-28T03:06:17Z","timestamp":1038452777000},"page":"557-566","source":"Crossref","is-referenced-by-count":21,"title":["The role of the sarcoplasmic reticulum in neonatal uterine smooth muscle: enhanced role compared to adult rat"],"prefix":"10.1113","volume":"545","author":[{"given":"Karen","family":"Noble","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Wray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.3.H930"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35038011"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001860102"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-97-4-898"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90009-A"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1996.76.4.967"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/eph8602181"},{"key":"e_1_2_5_9_1","first-page":"53","article-title":"Mg2+, ATP\u2010dependent Ca2+ transport in endoplasmic reticulum of myometrial cells","volume":"61","author":"Kosterin S. 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