{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:15:19Z","timestamp":1790702119633,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p>Recent studies have suggested that aquaporin\u20101 (AQP1) as well as the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>\u2010Cl<jats:sup>\u2212<\/jats:sup>transporter may be involved in CO<jats:sub>2<\/jats:sub>transport across biological membranes, but the physiological importance of this route of gas transport remained unknown. We studied CO<jats:sub>2<\/jats:sub>transport in human red blood cell ghosts at physiological temperatures (37 \u00b0C). Replacement of inert with CO<jats:sub>2<\/jats:sub>\u2010containing gas above a stirred cell suspension caused an outside\u2010to\u2010inside directed CO<jats:sub>2<\/jats:sub>gradient and generated a rapid biphasic intracellular acidification. The gradient of the acidifying gas was kept small to favour high affinity entry of CO<jats:sub>2<\/jats:sub>passing the membrane. All rates of acidification except that of the approach to physicochemical equilibrium of the uncatalysed reaction were restricted to the intracellular environment. Inhibition of carbonic anhydrase (CA) demonstrated that CO<jats:sub>2<\/jats:sub>\u2010induced acidification required the catalytic activity of CA. Blockade of the function of either AQP1 (by HgCl<jats:sub>2<\/jats:sub>at 65 \u03bcM) or the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>\u2010Cl<jats:sup>\u2212<\/jats:sup>transporter (by DIDS at 15 \u03bcM) completely prevented fast acidification. These data indicate that, at low chemical gradients for CO<jats:sub>2<\/jats:sub>, nearly the entire CO<jats:sub>2<\/jats:sub>transport across the red cell membrane is mediated by AQP1 and the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>\u2010Cl<jats:sup>\u2212<\/jats:sup>transporter. Therefore, these proteins may function as high affinity sites for CO<jats:sub>2<\/jats:sub>transport across the erythrocyte membrane.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.040113","type":"journal-article","created":{"date-parts":[[2003,5,20]],"date-time":"2003-05-20T01:48:20Z","timestamp":1053395300000},"page":"419-429","source":"Crossref","is-referenced-by-count":62,"title":["Aquaporin\u20101 and HCO<sub>3<\/sub><sup>\u2212<\/sup>\u2010Cl<sup>\u2212<\/sup>transporter\u2010mediated transport of CO<sub>2<\/sub>across the human erythrocyte membrane"],"prefix":"10.1113","volume":"550","author":[{"given":"Michael E.","family":"Blank","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heimo","family":"Ehmke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.102.1.99"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.6.C1481"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.023218"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37635-5"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(63)90042-0"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.155.3758.44"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013813"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15815"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.64.3.356"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.67.6.773"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(97)00068-0"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01868768"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.4.C1073"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(84)90366-3"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.2.C543"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(92)90269-R"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0005-2736(88)90276-3","article-title":"Sites of p\u2010chloromercuribenzene\u2010sulfonate inhibition of red cell urea and water transport","volume":"942","author":"Ojcius DM","year":"1988","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(88)90312-4"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.50.33123"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.256.5055.385"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54108-9"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7521540"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(72)90277-X"},{"key":"e_1_2_5_25_1","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1016\/S0021-9258(18)93538-6","article-title":"The protein of human erythrocyte membranes","volume":"243","author":"Rosenberg SA","year":"1968","journal-title":"J Biol Chem"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869522"},{"key":"e_1_2_5_27_1","first-page":"165","article-title":"Carbonic anhydrase: In the driver's seat for bicarbonate transport","volume":"2","author":"Sterling D","year":"2001","journal-title":"J Pancreas"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105959200"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02146936"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(86)90533-X"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.024398"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.43.28430"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/368332a0"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1981.tb06918.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.1999.00478.x"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(89)90532-4"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2686"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(92)90218-B"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2003.040113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2003.040113","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2003.040113","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2003.040113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T18:15:41Z","timestamp":1734027341000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2003.040113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1113\/jphysiol.2003.040113"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2003.040113","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}