{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:17:27Z","timestamp":1791901047612,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:p>\n                    We have examined the regulation of lipoprotein lipase (LPL) activity in skeletal muscle during physical inactivity in comparison to low\u2010intensity contractile activity of ambulatory controls. From studies acutely preventing ambulatory activity of one or both the hindlimbs in rats, it was shown that \u224890\u201395 % of the heparin\u2010releasable (HR) LPL activity normally present in rat muscle with ambulatory activity is lost, and thus dependent on local contractile activity. Similarly, \u224895 % of the differences in LPL activity between muscles of different fibre types was dependent on ambulatory activity. The robustness of the finding that physical inactivity significantly decreases muscle LPL activity was evident from confirmatory studies with different models of inactivity, in many rats and mice, both sexes, three muscle types and during both acute and chronic (11 days) treatment. Inactivity caused a local reduction of plasma [\n                    <jats:sup>3<\/jats:sup>\n                    H]triglyceride uptake into muscle and a decrease in high density lipoprotein cholesterol concentration. LPL mRNA was not differentially expressed between ambulatory controls and either the acutely or chronically inactive groups. Instead, the process involved a rapid loss of the HR\u2010LPL protein mass (the portion of LPL largely associated with the vascular endothelium) by an actinomycin D\u2010sensitive signalling mechanism (i.e. transcriptionally dependent process). Significant decreases of intracellular LPL protein content lagged behind the loss of HR\u2010LPL protein. Treadmill walking raised LPL activity \u22488\u2010fold (\n                    <jats:styled-content>\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.01\n                    <\/jats:styled-content>\n                    ) within 4 h after inactivity. The striking sensitivity of muscle LPL to inactivity and low\u2010intensity contractile activity may provide one piece of the puzzle for why inactivity is a risk factor for metabolic diseases and why even non\u2010vigorous activity provides marked protection against disorders involving poor lipid metabolism.\n                  <\/jats:p>","DOI":"10.1113\/jphysiol.2003.045591","type":"journal-article","created":{"date-parts":[[2003,8,29]],"date-time":"2003-08-29T21:50:26Z","timestamp":1062193826000},"page":"673-682","source":"Crossref","is-referenced-by-count":384,"title":["Suppression of skeletal muscle lipoprotein lipase activity during physical inactivity: a molecular reason to maintain daily low\u2010intensity activity"],"prefix":"10.1113","volume":"551","author":[{"given":"Lionel","family":"Bey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc T.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(94)90263-1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(87)90225-1"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00298.2002"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200325200"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00001.2002"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.91.2.687"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768-199911001-00025"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768-200106001-00002"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.89.3.1046"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000213272"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113354"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.2741\/Goldberg"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"S97","DOI":"10.1123\/ijsnem.11.s1.s97","article-title":"Plasma triglyceride metabolism in humans and rats during aging and physical inactivity","volume":"11","author":"Hamilton MT","year":"2001","journal-title":"Int J Sport Nutr Exerc Metab"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.88.1.327"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1998.275.6.E1016"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1249\/00005768-199406000-00001"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)32153-2"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/314164a0"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2001.24199"},{"key":"e_1_2_5_21_1","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1152\/jappl.1989.66.3.1093","article-title":"Transcriptional regulation of decreased protein synthesis during skeletal muscle unloading","volume":"66","author":"Howard G","year":"1989","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768-200106001-00003"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1056\/NEJM198112173052501","article-title":"Reduction of coronary atherosclerosis by moderate conditioning exercise in monkeys on an atherogenic diet","volume":"305","author":"Kramsch DM","year":"1981","journal-title":"N Eng J Med"},{"key":"e_1_2_5_24_1","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1152\/jappl.1991.71.2.404","article-title":"Regulation of lipoprotein lipase in muscle and adipose tissue during exercise","volume":"71","author":"Ladu MJ","year":"1991","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.27.17182"},{"key":"e_1_2_5_26_1","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1152\/ajplegacy.1976.231.3.860","article-title":"Lipoprotein lipase and uptake of chylomicrons triglyceride by skeletal muscle of rats","volume":"231","author":"Linder C","year":"1976","journal-title":"Am J Physiol"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(81)90059-7"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1981.tb06753.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1992.72.6.2210"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1980.49.5.851"},{"key":"e_1_2_5_31_1","first-page":"427","article-title":"Clearance of a chylomicron\u2010like emulsion from plasma is delayed in patients with coronary artery disease","volume":"28","author":"Martins MC","year":"1995","journal-title":"Braz J Med Biol Res"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(53)91495-0"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS19970072"},{"key":"e_1_2_5_34_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1152\/jappl.1990.69.1.58","article-title":"Rat hindlimb unloading: soleus histochemistry, ultrastructure, and electromyography","volume":"69","author":"Riley DA","year":"1990","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1997.272.2.E255"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.1.223"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(65)90109-8"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116813"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.90.3.954"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768-200106001-00012"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1060667"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.99.22.2901"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.1998.00303.x"},{"key":"e_1_2_5_44_1","first-page":"S82","article-title":"The effects of 20 days bed rest on serum lipids and lipoprotein concentrations in healthy young subjects","volume":"4","author":"Yanagibori R","year":"1997","journal-title":"J Gravit Physiol"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2003.045591","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2003.045591","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/jphysiol.2003.045591","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2003.045591","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T05:23:59Z","timestamp":1693805039000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2003.045591"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,9]]},"references-count":43,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1113\/jphysiol.2003.045591"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2003.045591","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,9]]}}}