{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T05:23:05Z","timestamp":1789968185488,"version":"4.0.1"},"reference-count":64,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>Exposure of the fetal sheep to moderate to severe hypoxic stress results in both increased cortical blood flow and decreased metabolic rate. Using intravenous infusion of 8\u2010cyclopentyl\u20101,3\u2010dipropylxanthine (DPCPX), a selective adenosine A<jats:sub>1<\/jats:sub> receptor antagonist that is permeable to the blood brain barrier, we examine the role of adenosine A<jats:sub>1<\/jats:sub> receptors in mediating cortical blood flow and metabolic responses to moderate hypoxia. The effects of DPCPX blockade are compared to controls as well as animals receiving intravenous 8\u2010(<jats:italic>p<\/jats:italic>\u2010sulfophenyl)\u2010theophylline) (8\u2010SPT), a non\u2010selective adenosine receptor antagonist which has been found to be blood brain barrier impermeable. Laser Doppler flow probes, tissue <jats:italic>P<\/jats:italic><jats:sub>O2<\/jats:sub>, and thermocouples were implanted in the cerebral cortices of near\u2010term fetal sheep. Catheters were placed in the brachial artery and sagittal sinus vein for collection of samples for blood gas analysis. Three to seven days later responses to a 30\u2010min period of fetal hypoxemia (arterial <jats:italic>P<\/jats:italic><jats:sub>O2<\/jats:sub> 10\u201312 mmHg) were studied with administration of 8\u2010SPT, DPCPX, or vehicle. Cerebral metabolic rate was determined by calculation of both brain heat production and oxygen consumption. In response to hypoxia, control experiments demonstrated a 42 \u00b1 7 % decrease in cortical heat production and a 35 \u00b1 10 % reduction in oxygen consumption. In contrast, DPCPX infusion during hypoxia resulted in no significant change in brain heat production or oxygen consumption, suggesting the adenosine A<jats:sub>1<\/jats:sub> receptor is involved in lowering metabolic rate during hypoxia. The decrease in cerebral metabolic rate was not altered by 8\u2010SPT infusion, suggesting that the response is not mediated by adenosine receptors located outside the blood brain barrier. In response to hypoxia, control experiments demonstrated a 35 \u00b1 7 % increase in cortical blood flow. DPCPX infusion did not change this increase in cortical blood flow, however 8\u2010SPT infusion attenuated increases in flow, indicating that hypoxic increases in cerebral blood flow are mediated by adenosine but not via the A<jats:sub>1<\/jats:sub> receptor. In summary, adenosine appears to play a key role in fetal hypoxic defences, acting to increase O<jats:sub>2<\/jats:sub> delivery via adenosine A<jats:sub>2<\/jats:sub> receptors and to decrease metabolic rate via A<jats:sub>1<\/jats:sub> receptors inside the blood brain barrier. These data show for the first time in the mammalian fetus that the adenosine A<jats:sub>1<\/jats:sub> receptor is an important mediator of brain metabolic rate during moderate hypoxia.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.047928","type":"journal-article","created":{"date-parts":[[2003,9,23]],"date-time":"2003-09-23T01:31:49Z","timestamp":1064280709000},"page":"935-945","source":"Crossref","is-referenced-by-count":59,"title":["Adenosine Mediates Decreased Cerebral Metabolic Rate and Increased Cerebral Blood Flow During Acute Moderate Hypoxia in the Near\u2010Term Fetal Sheep"],"prefix":"10.1113","volume":"553","author":[{"given":"Arlin B.","family":"Blood","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian J.","family":"Hunter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon 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