{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:43:05Z","timestamp":1791204185851,"version":"4.2.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>The amplitude of glycinergic miniature inhibitory postsynaptic currents (mIPSCs) varies considerably in neurons recorded in the isolated hindbrain of 50\u2010h\u2010old zebrafish larvae. At this age, glycinergic synapses are functionally mature. In order to measure the occupancy level of postsynaptic glycine receptors (GlyRs) and to determine the pre\u2010 and\/or postsynaptic origin of its variability, we analysed mIPSCs within bursts evoked by \u03b1\u2010latrotoxin (0.1\u20131 n<jats:sc>m<\/jats:sc>). Two types of burst were observed according to their mIPSC frequencies: \u2018slow\u2019 bursts with clearly spaced mIPSCs and \u2018fast\u2019 bursts characterised by superimposed events. Non\u2010stationary noise analysis of mIPSCs in some \u2018slow\u2019 bursts recorded in the presence or in the absence of Ca<jats:sup>2+<\/jats:sup> denoted that mIPSC amplitude variance did not depend on the quantity of neurotransmitters released (presynaptic origin), but rather on intrinsic stochastic behaviour of the same group of GlyRs (postsynaptic origin). In these bursts, the open probability measured at the peak of the mIPSCs was close to 0.5 while the maximum open probability is close to 0.9 for the synaptic isoform of GlyRs (heteromeric \u03b11\/\u03b2 GlyRs). In \u2018fast\u2019 bursts with superimposed events, a correlation was found between the amplitude of mIPSCs and the basal current level measured at their onset, which could suggest that the same group of GlyRs is activated during such bursts. Altogether, our results indicate that glycine synapses can display different release modes in the presence of \u03b1\u2010latrotoxin. They also indicate that, in our model, postsynaptic GlyRs cannot be saturated by the release of a single vesicle.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.049577","type":"journal-article","created":{"date-parts":[[2003,9,23]],"date-time":"2003-09-23T01:31:49Z","timestamp":1064280709000},"page":"819-832","source":"Crossref","is-referenced-by-count":21,"title":["Heterogeneity of Postsynaptic Receptor Occupancy Fluctuations among Glycinergic Inhibitory Synapses in the Zebrafish Hindbrain"],"prefix":"10.1113","volume":"553","author":[{"given":"Jean\u2010Michel","family":"Rigo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen 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