{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T22:51:52Z","timestamp":1762210312695},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>We tested the hypothesis that exercise\u2010induced changes in plasma composition result in peak stimulation of erythrocyte unidirectional K<jats:sup>+<\/jats:sup> (<jats:italic>J<\/jats:italic><jats:sub>K,in<\/jats:sub>) and net K<jats:sup>+<\/jats:sup> (<jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub>) transport within the first 120 s. In experimental series 1 (7 men; 2 women), plasma [K<jats:sup>+<\/jats:sup>] was continuously measured <jats:italic>in vitro<\/jats:italic> (37 \u00b0C) after the addition of red blood cells (RBCs) obtained from rested subjects (resting RBCs) into an exercise\u2010simulated plasma (ESP; increased plasma osmolality, [K<jats:sup>+<\/jats:sup>], [H<jats:sup>+<\/jats:sup>], [lactate] and [adrenaline] (epinephrine)), and <jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub> calculated. In experimental series 2 (7 men; 4 women), resting RBCs were incubated in true exercise plasma (TEP) obtained after two 30 s bouts of high intensity leg cycling exercise to determine <jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub> and <jats:italic>J<\/jats:italic><jats:sub>K,in<\/jats:sub> (via RBC <jats:sup>86<\/jats:sup>Rb accumulation). <jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub> of resting RBCs increased from 0.9 \u00b1 28.7 in resting plasma to 285 \u00b1 164 mmol (l RBCs)<jats:sup>\u22121<\/jats:sup> h<jats:sup>\u22121<\/jats:sup> in ESP and to 178 \u00b1 60 mmol (l RBCs)<jats:sup>\u22121<\/jats:sup> h<jats:sup>\u22121<\/jats:sup> after 10 s in TEP. Both <jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub> and <jats:italic>J<\/jats:italic><jats:sub>K,in<\/jats:sub> peaked within 10 s of incubation and decreased rapidly during the initial 120 s. The use of inhibitors for the Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase (ouabain) and the Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u20102Cl<jats:sup>\u2212<\/jats:sup> cotransporter (NKCC; bumetanide) indicated that rapid increases in <jats:italic>J<\/jats:italic><jats:sub>K,in<\/jats:sub> and <jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub> upon incubation of resting RBCs in TEP were due primarily to increased Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase activity; the NKCC appeared to be involved only when the Na<jats:sup>+<\/jats:sup>,K<jats:sup>+<\/jats:sup>\u2010ATPase was blocked. It is concluded that RBCs rapidly increase <jats:italic>J<\/jats:italic><jats:sub>K,in<\/jats:sub> and <jats:italic>J<\/jats:italic><jats:sub>K,net<\/jats:sub> in response to exercise\u2010induced changes in plasma composition.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.052860","type":"journal-article","created":{"date-parts":[[2003,10,7]],"date-time":"2003-10-07T00:26:26Z","timestamp":1065486386000},"page":"987-997","source":"Crossref","is-referenced-by-count":11,"title":["Exercise\u2010Induced Changes in Plasma Composition Increase Erythrocyte Na<sup>+<\/sup>,K<sup>+<\/sup>\u2010ATPase, but not Na<sup>+<\/sup>\u2013K<sup>+<\/sup>\u20132Cl<sup>\u2212<\/sup> Cotransporter, Activity to Stimulate net and Unidirectional K<sup>+<\/sup> Transport in Humans"],"prefix":"10.1113","volume":"553","author":[{"given":"Michael I.","family":"Lindinger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon 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