{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:17:28Z","timestamp":1777605448988,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,12,10]],"date-time":"2003-12-10T00:00:00Z","timestamp":1071014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p>In the paraventricular nucleus (PVN) of the hypothalamus, nitric oxide (NO) inhibits sympathetic outflow through increased GABA release. However, the signal transduction pathways involved in its action remain unclear. In the present study, we determined the role of cGMP, soluble guanylyl cyclase, and protein kinase G in the potentiating effect of NO on synaptic GABA release to spinally projecting PVN neurones. The PVN neurones were retrogradely labelled by a fluorescent tracer injected into the thoracic spinal cord of rats. Whole\u2010cell voltage\u2010clamp recordings were performed on labelled PVN neurones in the hypothalamic slice. Bath application of the NO donor, <jats:italic>S<\/jats:italic>\u2010nitroso\u2010<jats:italic>N<\/jats:italic>\u2010acetyl\u2010penicillamine (SNAP), reproducibly increased the frequency of miniature GABAergic inhibitory postsynaptic currents (mIPSCs) without changing the amplitude and the decay time constant. Neither replacement of Ca<jats:sup>2+<\/jats:sup> with Co<jats:sup>2+<\/jats:sup> nor application of Cd<jats:sup>2+<\/jats:sup> to block the Ca<jats:sup>2+<\/jats:sup> channel altered the effect of SNAP on mIPSCs. Also, the effect of SNAP on mIPSCs was not significantly affected by thapsigargin, a Ca<jats:sup>2+<\/jats:sup>\u2010ATPase inhibitor that depletes intracellular Ca<jats:sup>2+<\/jats:sup> stores. Application of a membrane\u2010permeant cGMP analogue, pCPT\u2013cGMP, mimicked the effect of SNAP on mIPSCs in the presence of a phosphodiesterase inhibitor, IBMX. Furthermore, both the soluble guanylyl cyclase inhibitor, ODQ, and the specific protein kinase G inhibitor, Rp pCPT cGMP, abolished the effect of SNAP on mIPSCs. Thus, these data provide substantial new information that NO potentiates GABAergic synaptic inputs to spinally projecting PVN neurones through a cGMP\u2013protein kinase G pathway.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.053371","type":"journal-article","created":{"date-parts":[[2003,10,14]],"date-time":"2003-10-14T00:28:18Z","timestamp":1066091298000},"page":"100-110","source":"Crossref","is-referenced-by-count":87,"title":["Signalling pathway of nitric oxide in synaptic GABA release in the rat paraventricular nucleus"],"prefix":"10.1113","volume":"554","author":[{"given":"De\u2010Pei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao\u2010Rui","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas 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