{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:31:48Z","timestamp":1790245908447,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,1]],"date-time":"2003-12-01T00:00:00Z","timestamp":1070236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>Ca<jats:sup>2+<\/jats:sup> liberation through inositol 1,4,5\u2010trisphosphate receptors (IP<jats:sub>3<\/jats:sub>Rs) plays a universal role in cell regulation, and specificity of cell signalling is achieved through the spatiotemporal patterning of Ca<jats:sup>2+<\/jats:sup> signals. IP<jats:sub>3<\/jats:sub>Rs display Ca<jats:sup>2+<\/jats:sup>\u2010induced Ca<jats:sup>2+<\/jats:sup> release (CICR), but are grouped in clusters so that regenerative Ca<jats:sup>2+<\/jats:sup> signals may remain localized to individual clusters, or propagate globally between clusters by successive cycles of Ca<jats:sup>2+<\/jats:sup> diffusion and CICR. We used confocal microscopy and photoreleased IP<jats:sub>3<\/jats:sub> in <jats:italic>Xenopus<\/jats:italic> oocytes to study how these properties are modulated by mobile cytosolic Ca<jats:sup>2+<\/jats:sup> buffers. EGTA (a buffer with slow \u2018on\u2010rate\u2019) speeded Ca<jats:sup>2+<\/jats:sup> signals and \u2018balkanized\u2019 Ca<jats:sup>2+<\/jats:sup> waves by dissociating them into local signals. In contrast, BAPTA (a fast buffer with similar affinity) slowed Ca<jats:sup>2+<\/jats:sup> responses and promoted \u2018globalization\u2019 of spatially uniform Ca<jats:sup>2+<\/jats:sup> signals. These actions are likely to arise through differential effects on Ca<jats:sup>2+<\/jats:sup> feedback within and between IP<jats:sub>3<\/jats:sub>R clusters, because Ca<jats:sup>2+<\/jats:sup> signals evoked by influx through voltage\u2010gated channels were little affected. We propose that cell\u2010specific expression of Ca<jats:sup>2+<\/jats:sup>\u2010binding proteins with distinct kinetics may shape the time course and spatial distribution of IP<jats:sub>3<\/jats:sub>\u2010evoked Ca<jats:sup>2+<\/jats:sup> signals for specific physiological roles.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.054247","type":"journal-article","created":{"date-parts":[[2003,10,14]],"date-time":"2003-10-14T00:28:18Z","timestamp":1066091298000},"page":"775-788","source":"Crossref","is-referenced-by-count":126,"title":["Buffer Kinetics Shape the Spatiotemporal Patterns of IP<b><sub>3<\/sub><\/b>\u2010Evoked Ca<sup>2+<\/sup> Signals"],"prefix":"10.1113","volume":"553","author":[{"given":"Sheila 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