{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:24:33Z","timestamp":1760297073945},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T00:00:00Z","timestamp":1081814400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:p>The activation properties of GABA<jats:sub>A<\/jats:sub> receptors containing \u03b14\u03b22\u03b32 and \u03b14\u03b22\u03b4 subunits were examined in the presence of GABA or pentobarbital. The receptors were expressed transiently in HEK 293 cells, and the electrophysiological experiments were carried out using cell\u2010attached single\u2010channel patch clamp or whole\u2010cell macroscopic recordings. The data show that GABA is a stronger activator of \u03b14\u03b22\u03b32 receptors than \u03b14\u03b22\u03b4 receptors. Single\u2010channel clusters were recorded from \u03b14\u03b22\u03b32 receptors in the presence of 10\u20135000 \u03bc<jats:sc>m<\/jats:sc> GABA. The maximal intracluster open probability was 0.35, with a half\u2010maximal response elicited by 32 \u03bc<jats:sc>m<\/jats:sc> GABA. Simultaneous kinetic analysis of single\u2010channel currents obtained at various GABA concentrations yields a channel opening rate constant of 250 s<jats:sup>\u22121<\/jats:sup>, and a <jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub> of 20 \u03bc<jats:sc>m<\/jats:sc>. In contrast, only isolated openings were observed in the presence of GABA for the \u03b14\u03b22\u03b4 receptor. Pentobarbital was a strong activator of both \u03b14\u03b22\u03b32 and \u03b14\u03b22\u03b4 receptors. The maximal cluster open probability, recorded in the presence of 100 \u03bc<jats:sc>m<\/jats:sc> pentobarbital, was 0.7. At higher pentobarbital concentrations, the cluster open probability was reduced, probably due to channel block. The results from single\u2010channel experiments were confirmed by macroscopic recordings from HEK cells in the presence of GABA or pentobarbital.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.058230","type":"journal-article","created":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T01:35:54Z","timestamp":1076981754000},"page":"387-399","source":"Crossref","is-referenced-by-count":54,"title":["Activation of GABA<sub>A<\/sub> receptors containing the \u03b14 subunit by GABA and pentobarbital"],"prefix":"10.1113","volume":"556","author":[{"given":"Gustav","family":"Akk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Bracamontes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe 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