{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:16:58Z","timestamp":1790857018936,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T00:00:00Z","timestamp":1081814400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:p>Ca<jats:sup>2+<\/jats:sup>\u2010binding proteins (CaBPs) are expressed in a highly specific manner across many different cell types, yet the physiological basis underlying their selective distribution patterns remains unclear. We used confocal line\u2010scan microscopy together with photo\u2010release of IP<jats:sub>3<\/jats:sub> in <jats:italic>Xenopus<\/jats:italic> oocytes to investigate the actions of mobile cytosolic CaBPs on the spatiotemporal properties of IP<jats:sub>3<\/jats:sub>\u2010evoked Ca<jats:sup>2+<\/jats:sup> signals. Parvalbumin (PV), a CaBP with slow Ca<jats:sup>2+<\/jats:sup>\u2010binding kinetics, shortened the duration of IP<jats:sub>3<\/jats:sub>\u2010evoked Ca<jats:sup>2+<\/jats:sup> signals and \u2018balkanized\u2019 global responses into discrete localized events (puffs). In contrast, calretinin (CR), a presumed fast buffer, prolonged Ca<jats:sup>2+<\/jats:sup> responses and promoted \u2018globalization\u2019 of spatially uniform Ca<jats:sup>2+<\/jats:sup> signals at high [IP<jats:sub>3<\/jats:sub>]. Oocytes loaded with CR or PV showed Ca<jats:sup>2+<\/jats:sup> puffs following photolysis flashes that were subthreshold in controls, and the spatiotemporal properties of these localized events were differentially modulated by PV and CR. In comparison to results we previously obtained with exogenous Ca<jats:sup>2+<\/jats:sup> buffers, PV closely mimicked the actions of the slow buffer EGTA, whereas CR showed important differences from the fast buffer BAPTA. Most notably, puffs were never observed after loading BAPTA, and this exogenous buffer did not show the marked sensitization of IP<jats:sub>3<\/jats:sub> action evident with CR. The ability of Ca<jats:sup>2+<\/jats:sup> buffers and CaBPs with differing kinetics to fine\u2010tune both global and local intracellular Ca<jats:sup>2+<\/jats:sup> signals is likely to have significant physiological implications.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.059204","type":"journal-article","created":{"date-parts":[[2004,2,3]],"date-time":"2004-02-03T01:23:15Z","timestamp":1075771395000},"page":"447-461","source":"Crossref","is-referenced-by-count":79,"title":["Spatiotemporal patterning of IP<sub>3<\/sub>\u2010mediated Ca<sup>2+<\/sup> signals in <i>Xenopus<\/i> oocytes by Ca<sup>2+<\/sup>\u2010binding proteins"],"prefix":"10.1113","volume":"556","author":[{"given":"Sheila 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