{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:56:18Z","timestamp":1790384178344,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,5,24]],"date-time":"2004-05-24T00:00:00Z","timestamp":1085356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> currents were studied in inner hair cells (IHCs) of mature mice. <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub>, the large\u2010conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> current (BK) characteristic of mature IHCs, had a fast activation time constant (0.4 ms at \u221225 mV at room temperature) and did not inactivate during 170 ms. Its amplitude, measured at \u221225 mV, and activation time constant were similar between IHCs in the apical and basal regions of the cochlea. <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub> was selectively blocked by 30 n<jats:sc>m<\/jats:sc> IbTx but was unaffected by superfusion of Ca<jats:sup>2+<\/jats:sup>\u2010free solution, nifedipine or Bay K 8644, excluding the direct involvement of voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> channels in <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub> activation. Increasing the intracellular concentration of the Ca<jats:sup>2+<\/jats:sup> chelator BAPTA from 0.1 m<jats:sc>m<\/jats:sc> to 30 m<jats:sc>m<\/jats:sc> reduced the amplitude of <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub> at \u221225 mV and shifted its activation by 37 mV towards more depolarized potentials. A reduction in the size of <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub> and a depolarizing shift of its activation were also seen when either thapsigargin and caffeine or ryanodine were added intracellularly, suggesting that <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub> is modulated by voltage\u2010dependent release from intracellular Ca<jats:sup>2+<\/jats:sup> stores. Mature IHCs had a small additional Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> current (<jats:italic>I<\/jats:italic><jats:sub>K(Ca)<\/jats:sub>), activated by Ca<jats:sup>2+<\/jats:sup> flowing through L\u2010type Ca<jats:sup>2+<\/jats:sup> channels. This current was still present during superfusion of either IbTx (60 n<jats:sc>m<\/jats:sc>) or apamin (300 n<jats:sc>m<\/jats:sc>) but was abolished in Cs<jats:sup>+<\/jats:sup>\u2010based intracellular solution or during superfusion of 5 m<jats:sc>m<\/jats:sc> TEA, suggesting the presence of an additional BK\u2010channel type. Current clamp experiments at body temperature show that <jats:italic>I<\/jats:italic><jats:sub>K,f<\/jats:sub>, but not <jats:italic>I<\/jats:italic><jats:sub>K(Ca)<\/jats:sub>, is essential for fast voltage responses of mature IHCs.<\/jats:p>","DOI":"10.1113\/jphysiol.2003.060137","type":"journal-article","created":{"date-parts":[[2004,4,6]],"date-time":"2004-04-06T00:44:11Z","timestamp":1081212251000},"page":"613-633","source":"Crossref","is-referenced-by-count":85,"title":["Effects of intracellular stores and extracellular Ca<sup>2+<\/sup> on Ca<sup>2+<\/sup>\u2010activated K<sup>+<\/sup> currents in mature mouse inner hair cells"],"prefix":"10.1113","volume":"557","author":[{"given":"Walter","family":"Marcotti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart L.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corn\u00e9 J.","family":"Kros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,5,24]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"147","article-title":"Single\u2010channel recordings of large conductance Ca2+\u2010activated K+ channels from guinea\u2010pig inner hair cells","volume":"501","author":"Appenrodt P","year":"1997","journal-title":"J Physiol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00049.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016492"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019850"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107118200"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.51.3.279"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.85"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00373911"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-15-04543.1996"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00612025"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5955(94)90159-7"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018271"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1523\/JNEUROSCI.08-07-02460.1988","article-title":". 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