{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:05:25Z","timestamp":1767650725983},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,6,14]],"date-time":"2004-06-14T00:00:00Z","timestamp":1087171200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>Hyaluronan (HA), a component of synovial fluid, buffers fluid loss from joints. To explain this, a quantitative theory for HA concentration polarisation at a partially sieving synovial lining was developed. The theory predicts a fall in HA reflected fraction <jats:italic>R<\/jats:italic> with increased filtration rate. To test this, knees of anaesthetised rabbits were infused with HA and fluorescein\u2013dextran (FD) at constant trans\u2010synovial filtration rates of 6\u201389 \u03bcl min<jats:sup>\u22121<\/jats:sup>. Samples of femoral lymph, mixed intra\u2010articular fluid and subsynovial fluid after \u2265 3 h were analysed by high\u2010performance liquid chromatography. <jats:italic>R<\/jats:italic> was calculated as (1 \u2013 downstream\/upstream concentration), using [FD] to adjust for joint lymph dilution in femoral lymph. Intra\u2010articular HA concentration after \u2265 3 h, 0.47 \u00b1 0.02 mg ml<jats:sup>\u22121<\/jats:sup> (mean \u00b1<jats:sc>s.e.m.<\/jats:sc>, <jats:italic>n<\/jats:italic>= 31), exceeded the infusate concentration, 0.20 mg ml<jats:sup>\u22121<\/jats:sup>, while subsynovial and lymph [HA] were reduced relative to [FD]. The changes in [HA] demonstrated synovial molecular sieving of HA. <jats:italic>R<\/jats:italic> from cavity to lymph (<jats:italic>R<\/jats:italic><jats:sub>lymph<\/jats:sub>) fell monotonically from 0.93 at 6 \u03bcl min<jats:sup>\u22121<\/jats:sup> to 0.14 at 89 \u03bcl min<jats:sup>\u22121<\/jats:sup> (<jats:italic>P<\/jats:italic> &lt; 0.0001, regression analysis, <jats:italic>n<\/jats:italic>= 33). <jats:italic>R<\/jats:italic> values calculated from the intra\u2010articular HA accumulation (<jats:italic>R<\/jats:italic><jats:sub>asp<\/jats:sub>) or the low subsynovial concentrations (<jats:italic>R<\/jats:italic><jats:sub>syn<\/jats:sub>) were similar negative functions of filtration rate. <jats:italic>R<\/jats:italic> for lymphatic capillary endothelium (<jats:italic>R<\/jats:italic><jats:sub>endo<\/jats:sub>), calculated from lymph\/subsynovial concentration ratios, was effectively zero (\u22120.03 \u00b1 0.18, <jats:italic>n<\/jats:italic>= 21), confirming that synovium, not initial lymphatic endothelium, is the reflection site. Logarithmic linearisation of the results evaluated the synovial HA reflection coefficient as 0.91. In conclusion, the existence of concentration polarisation during joint fluid drainage was supported by the demonstration of a negative relation between filtration rate and <jats:italic>R<\/jats:italic><jats:sub>lymph<\/jats:sub>, <jats:italic>R<\/jats:italic><jats:sub>asp<\/jats:sub> and <jats:italic>R<\/jats:italic><jats:sub>syn<\/jats:sub>.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.063529","type":"journal-article","created":{"date-parts":[[2004,4,13]],"date-time":"2004-04-13T00:34:31Z","timestamp":1081816471000},"page":"909-922","source":"Crossref","is-referenced-by-count":14,"title":["Filtration rate dependence of hyaluronan reflection by joint\u2010to\u2010lymph barrier: evidence for concentration polarisation"],"prefix":"10.1113","volume":"557","author":[{"given":"S.","family":"Sabaratnam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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