{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T22:42:06Z","timestamp":1778020926854,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,6,24]],"date-time":"2004-06-24T00:00:00Z","timestamp":1088035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p>Light depolarizes retinal On bipolar cells, opening the cation\u2010selective channels that are responsible for producing the synaptic current. In this study, the basic features of light\u2010induced signals were mimicked by bathing slices of salamander retina with an agonist for the mGluR6 receptor that is expressed on the dendrites of On cells, and then displacing the agonist with the mGluR6 antagonist (<jats:italic>RS<\/jats:italic>)\u2010<jats:italic>a<\/jats:italic>\u2010cyclopropyl\u20104\u2010phosphonophenylglycine (CPPG). The transduction current that is activated by this protocol rapidly shuts off, or desensitizes. Desensitization was highly correlated with the concentration and the type of Ca<jats:sup>2+<\/jats:sup> buffer that was dialysed into the cell: When Ca<jats:sup>2+<\/jats:sup> buffering was minimized by dialysing cells with 0.5 m<jats:sc>m<\/jats:sc> EGTA, the steady\u2010state response was reduced to approximately 40% of the peak response. Buffering with 10 m<jats:sc>m<\/jats:sc> EGTA reduced desensitization, while BAPTA completely eliminated it. Removing external Ca<jats:sup>2+<\/jats:sup> also prevented desensitization, suggesting that entry of\u2003 Ca<jats:sup>2+<\/jats:sup> through the transduction channel provides the trigger. The time course of desensitization was measured by using a voltage jump protocol to rapidly increase Ca<jats:sup>2+<\/jats:sup> influx, and could be fitted with a single time constant on the order of 1 s, in good agreement with previously published rates of desensitization to steps of light in this species. It is proposed that Ca<jats:sup>2+<\/jats:sup>\u2010dependent shut\u2010off of the On bipolar cell transduction current may contribute to the conversion of sustained to transient light responses that predominate in the inner retina.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.064980","type":"journal-article","created":{"date-parts":[[2004,5,18]],"date-time":"2004-05-18T00:44:06Z","timestamp":1084841046000},"page":"137-146","source":"Crossref","is-referenced-by-count":37,"title":["Desensitization of the mGluR6 transduction current in tiger salamander On bipolar 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