{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:39:41Z","timestamp":1790206781617,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,8,10]],"date-time":"2004-08-10T00:00:00Z","timestamp":1092096000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>Previous reports suggest that an important characteristic of chemosensitive neurones is an unusually large change of steady\u2010state intracellular pH in response to a change in extracellular pH (\u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub>). To determine whether such a correlation exists between neurones from the medullary raphe (a chemosensitive brain region) and hippocampus (a non\u2010chemosensitive region), we used BCECF to monitor pH<jats:sub>i<\/jats:sub> in cultured neurones subjected to extracellular acid\u2013base disturbances. In medullary raphe neurones, respiratory acidosis (5%\u2192 9% CO<jats:sub>2<\/jats:sub>) caused a rapid fall in pH<jats:sub>i<\/jats:sub> (\u0394pH<jats:sub>i<\/jats:sub>\u223c0.2) with no recovery and a large \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub> of 0.71. Hippocampal neurones had a similar response, but with a slightly lower \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub> (0.59). We further investigated a possible link between pH<jats:sub>i<\/jats:sub> regulation and chemosensitivity by following the pH<jats:sub>i<\/jats:sub> measurements on medullary raphe neurones with an immunocytochemistry for tryptophan hydroxylase (a marker of serotonergic neurones). We found that the \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub> of 0.69 for serotonergic neurones (which are stimulated by acidosis) was not different from either the \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub> of 0.75 for non\u2010serotonergic neurones (most of which are not chemosensitive), or from the \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub> of hippocampal neurones. For both respiratory alkalosis (5%\u2192 3% CO<jats:sub>2<\/jats:sub>) and metabolic alkalosis (22 m<jats:sc>m<\/jats:sc>\u2192 35 m<jats:sc>m<\/jats:sc> HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>), \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub> was 0.42\u20130.53 for all groups of neurones studied. The only notable difference between medullary raphe and hippocampal neurones was in response to metabolic acidosis (22 m<jats:sc>m<\/jats:sc>\u2192 14 m<jats:sc>m<\/jats:sc> HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>), which caused a large pH<jats:sub>i<\/jats:sub> decrease in \u223c80% of medullary raphe neurones (\u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub>= 0.71), but relatively little pH<jats:sub>i<\/jats:sub> decrease in 70% of the hippocampal neurones (\u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub>= 0.09). Our comparison of medullary raphe and hippocampal neurones indicates that, except in response to metabolic acidosis, the neurones from the chemosensitive region do not have a uniquely high \u0394pH<jats:sub>i<\/jats:sub>\/\u0394pH<jats:sub>o<\/jats:sub>. Moreover, regardless of whether neurones were cultured from the chemosensitive or the non\u2010chemosensitive region, pH<jats:sub>i<\/jats:sub> did not recover during any of the acid\u2013base stresses.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.067793","type":"journal-article","created":{"date-parts":[[2004,6,15]],"date-time":"2004-06-15T18:22:31Z","timestamp":1087323751000},"page":"85-101","source":"Crossref","is-referenced-by-count":50,"title":["Effect of extracellular acid\u2013base disturbances on the intracellular pH of neurones cultured from rat medullary raphe or hippocampus"],"prefix":"10.1113","volume":"559","author":[{"given":"Patrice","family":"Bouyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania 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