{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T05:25:02Z","timestamp":1770355502313,"version":"3.49.0"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,8,25]],"date-time":"2004-08-25T00:00:00Z","timestamp":1093392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:p>SPOC1 airway goblet cells secrete mucin in response to P2Y<jats:sub>2<\/jats:sub> receptor agonists and to secretagogues, phorbol 12\u2010myristate 13\u2010acetate (PMA) and ionomycin, which mobilize elements of the phospholipase C pathway, PKC and Ca<jats:sup>2+<\/jats:sup>, respectively. Previous studies demonstrated that mucin secretion from SLO\u2010permeabilized, EGTA\u2010buffered SPOC1 cells was stimulated by PMA at low Ca<jats:sup>2+<\/jats:sup> levels (&lt; 0.1 \u03bc<jats:sc>m<\/jats:sc>), consistent with the notion that regulated exocytosis may occur by Ca<jats:sup>2+<\/jats:sup>\u2010independent pathways. We tested the alternative hypothesis that PMA\u2010induced mucin secretion is, in fact, a Ca<jats:sup>2+<\/jats:sup>\u2010dependent process under the conditions of low bulk Ca<jats:sup>2+<\/jats:sup>, one that is permitted in the typical SLO\u2010permeabilized cell model by the slow binding kinetics of EGTA. Both IP<jats:sub>3<\/jats:sub> and elevated bulk Ca<jats:sup>2+<\/jats:sup> activated mucin secretion in SPOC1 cells buffered by EGTA, suggesting that IP<jats:sub>3<\/jats:sub> generates a local Ca<jats:sup>2+<\/jats:sup> gradient in the vicinity of the secretory granules to the degree necessary to trigger exocytosis. BAPTA, which binds Ca<jats:sup>2+<\/jats:sup> approximately 100\u2010fold faster than EGTA, diminished IP<jats:sub>3<\/jats:sub>\u2010induced mucin release over a range of concentrations by \u2265 69%, yet maintained an essentially normal mucin secretory response to elevated bulk Ca<jats:sup>2+<\/jats:sup> in permeabilized SPOC1 cells. BAPTA also diminished the mucin secretory response of permeabilized cells to PMA, relative to the EGTA\u2010buffered control: at PMA below 30 n<jats:sc>m<\/jats:sc>, BAPTA abolished the secretory response, and at higher concentrations it was reduced significantly relative to the EGTA\u2010buffered controls. PMA\u2010induced secretion in EGTA was insensitive to heparin. These results suggest that Ca<jats:sup>2+<\/jats:sup> is released locally during PMA\u2010induced exocytosis, by an IP<jats:sub>3<\/jats:sub>\u2010independent mechanism.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.070433","type":"journal-article","created":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T00:25:10Z","timestamp":1088123110000},"page":"555-565","source":"Crossref","is-referenced-by-count":26,"title":["Ca<sup>2+<\/sup> dependency of \u2018Ca<sup>2+<\/sup>\u2010independent\u2019 exocytosis in SPOC1 airway goblet cells"],"prefix":"10.1113","volume":"559","author":[{"given":"Andrea H.","family":"Rossi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick R.","family":"Sears","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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