{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:48:51Z","timestamp":1789865331507,"version":"4.0.1"},"reference-count":68,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T00:00:00Z","timestamp":1105056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,1,15]]},"abstract":"<jats:p>The Na<jats:sup>+<\/jats:sup>\u2013HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> cotransporter (NBC) is an important sarcolemmal acid extruder in cardiac muscle. The characteristics of NBC expressed functionally in heart are controversial, with reports suggesting electroneutral (NBCn; 1HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> : 1Na<jats:sup>+<\/jats:sup>; coupling coefficient <jats:italic>N<\/jats:italic>= 1) or electrogenic forms of the transporter (NBCe; equivalent to 2HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> : 1Na<jats:sup>+<\/jats:sup>; <jats:italic>N<\/jats:italic>= 2). We have used voltage\u2010clamp and epifluorescence techniques to compare NBC activity in isolated ventricular myocytes from rabbit, rat and guinea pig. Depolarization (by voltage clamp or hyperkalaemia) reversibly increased steady\u2010state pH<jats:sub>i<\/jats:sub> while hyperpolarization decreased it, effects seen only in CO<jats:sub>2<\/jats:sub>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>\u2010buffered solutions, and blocked by S0859 (cardiac NBC inhibitor). Species differences in amplitude of these pH<jats:sub>i<\/jats:sub> changes were rat &gt;\u2003guinea\u2003pig\u2003\u2248 rabbit. Tonic depolarization (\u2212140 mV to \u22120 mV) accelerated NBC\u2010mediated pH<jats:sub>i<\/jats:sub> recovery from an intracellular acid load. At 0 mV, NBC\u2010mediated outward current at resting pH<jats:sub>i<\/jats:sub> was +0.52 \u00b1 0.05 pA pF<jats:sup>\u22121<\/jats:sup> (rat, <jats:italic>n<\/jats:italic>= 5), +0.26 \u00b1 0.05 pA pF<jats:sup>\u22121<\/jats:sup> (guinea pig, <jats:italic>n<\/jats:italic>= 5) and +0.10 \u00b1 0.03 pA pF<jats:sup>\u22121<\/jats:sup> (rabbit, <jats:italic>n<\/jats:italic>= 9), with reversal potentials near \u2212100 mV, consistent with <jats:italic>N<\/jats:italic>= 2. The above results indicate a functionally active voltage\u2010sensitive NBCe in these species. Voltage\u2010clamp hyperpolarization negative to the reversal potential for NBCe failed, however, to terminate or reverse NBC\u2010mediated pH<jats:sub>i<\/jats:sub>\u2010recovery from an acid load although it was slowed significantly, suggesting electroneutral NBC may also be operational. NBC\u2010mediated pH<jats:sub>i<\/jats:sub> recovery was associated with a rise of [Na<jats:sup>+<\/jats:sup>]<jats:sub>i<\/jats:sub> at a rate \u223c25% of that mediated via NHE, and consistent with an apparent NBC stoichiometry between <jats:italic>N<\/jats:italic>= 1 and <jats:italic>N<\/jats:italic>= 2. In conclusion, NBCe in the ventricular myocyte displays considerable functional variation among the three species tested (greatest in rat, least in rabbit) and may coexist with some NBCn activity.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.071068","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T01:13:25Z","timestamp":1100826805000},"page":"455-475","source":"Crossref","is-referenced-by-count":67,"title":["Functional diversity of electrogenic Na<sup>+<\/sup>\u2013HCO<sub>3<\/sub><sup>\u2212<\/sup> cotransport in ventricular myocytes from rat, rabbit and guinea pig"],"prefix":"10.1113","volume":"562","author":[{"given":"Taku","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel","family":"Swietach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Rossini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Hurng","family":"Loh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Vaughan\u2010Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth 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