{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:25:04Z","timestamp":1791498304601,"version":"4.3.4"},"reference-count":69,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,29]],"date-time":"2004-10-29T00:00:00Z","timestamp":1099008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:p>From just after birth, mouse inner hair cells (IHCs) expressed a Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> current that was reduced by intracellular BAPTA at concentrations \u2265 1 m<jats:sc>m<\/jats:sc>. The block of this current by nifedipine suggests the direct involvement of Ca<jats:sub>v<\/jats:sub>1.3 Ca<jats:sup>2+<\/jats:sup> channels in its activation. On the basis of its high sensitivity to apamin (<jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub> 360 p<jats:sc>m<\/jats:sc>) it was identified as a small\u2010conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> current (SK), probably SK2. A similar current was also found in outer hair cells (OHCs) from the beginning of the second postnatal week. In both cell types the appearance of the SK current coincided with their becoming responsive to acetylcholine (ACh), the main efferent neurotransmitter in the cochlea. The effect of ACh on IHCs was abolished when they were simultaneously superfused with strychnine, consistent with the presence of nicotinic ACh receptors (nAChRs). Extracellular Ca<jats:sup>2+<\/jats:sup> either potentiated or blocked the nAChR current depending on its concentration, as previously reported for the recombinant \u03b19\u03b110 nAChR. Outward currents activated by ACh were reduced by blocking the SK current with apamin or by preventing SK current activation with intracellular BAPTA (\u2265 10 m<jats:sc>m<\/jats:sc>). The endogenous mobile Ca<jats:sup>2+<\/jats:sup> buffer concentration was estimated to be equivalent to about 1 m<jats:sc>m<\/jats:sc> BAPTA, suggesting that in physiological conditions the SK channel is significantly activated by Ca<jats:sup>2+<\/jats:sup> influx through both Ca<jats:sub>v<\/jats:sub>1.3 Ca<jats:sup>2+<\/jats:sup> channels and \u03b19\u03b110 nAChRs. Current clamp experiments showed that in IHCs the SK current is required for sustaining a train of action potentials and also modulates their frequency when activated by ACh.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.072868","type":"journal-article","created":{"date-parts":[[2004,8,27]],"date-time":"2004-08-27T00:13:00Z","timestamp":1093565580000},"page":"691-708","source":"Crossref","is-referenced-by-count":115,"title":["A transiently expressed SK current sustains and modulates action potential activity in immature mouse inner hair cells"],"prefix":"10.1113","volume":"560","author":[{"given":"Walter","family":"Marcotti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart L.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corn\u00e9 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