{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:50:45Z","timestamp":1790985045065,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T00:00:00Z","timestamp":1106179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p>The rhabdomyosarcoma cell line (SJ\u2010RH30) exhibits both ultrastructural and electrophysiological hallmarks of mammalian skeletal muscle. We have used patch\u2010clamp electrophysiology to study acid\u2010gated currents in these cells. At a holding potential of \u221260 mV, rapid application of extracellular solutions of pH 6.5 produced inward current responses in \u223c85% of cells. The amplitude of these responses exhibited a marked pH dependence. In addition, the kinetics of both activation and desensitization were faster at more acidic pH, whereas the deactivation rate was pH independent. Repeated applications of a pH 6.0 solution produced a tachyphalaxis that could be substantially attenuated by reducing the duration of the acid challenge and increasing the interstimulus interval. The current\u2013voltage relationship of the acid\u2010induced currents was linear at positive potentials but an area of negative slope conductance was observed in the negative potential range. This was not eliminated by removal of extracellular Ca<jats:sup>2+<\/jats:sup>, a manoeuvre which did, however, substantially increase current amplitude. Changing the transmembrane Na<jats:sup>+<\/jats:sup> gradient altered the current\u2013voltage relationship in a fashion commensurate with an underlying conductance predominantly permeable to Na<jats:sup>+<\/jats:sup>. Pharmacologically, acid\u2010induced currents were blocked 84.4 \u00b1 1.2% by 30 \u03bc<jats:sc>m<\/jats:sc> amiloride and 91.8 \u00b1 3.0% by a 1 : 1000 dilution of <jats:italic>Psalmopoeus<\/jats:italic><jats:italic>cambridgei<\/jats:italic> venom. Inhibition by both agents could be reversed by a short period of compound washout. By contrast, APETx2 had no significant effect on acid\u2010evoked currents. These observations strongly suggest the acid\u2010induced current is mediated by ASIC1 channels. In agreement with this, current responses of SJ\u2010RH30 cells to a pH 6.0 challenge were greatly enhanced by extracellular lactate. These data demonstrate the presence of ASIC1 channels in a cell line with skeletal muscle characteristics. In addition, significant levels of ASIC1 and ASIC3 mRNA were found in skeletal muscle tissue samples. These findings are discussed with regard to the role such a conductance might play if present in skeletal muscle <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.075069","type":"journal-article","created":{"date-parts":[[2004,12,3]],"date-time":"2004-12-03T01:29:03Z","timestamp":1102037343000},"page":"759-769","source":"Crossref","is-referenced-by-count":45,"title":["Functional properties and pharmacological inhibition of ASIC channels in the human SJ\u2010RH30 skeletal muscle cell line"],"prefix":"10.1113","volume":"562","author":[{"given":"D. P.","family":"Gitterman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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