{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:38:36Z","timestamp":1790642316851,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T00:00:00Z","timestamp":1105056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,1,15]]},"abstract":"<jats:p>We have defined Ca<jats:sup>2+<\/jats:sup> channel subtypes expressed in rabbit carotid body (CB) chemoreceptor cells and their participation in the stimulus\u2010evoked catecholamine (CA) release. Ca<jats:sup>2+<\/jats:sup> currents (<jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>) activated at \u201330 mV, peaked at +10 mV and were fully blocked by 200 \u03bc<jats:sc>m<\/jats:sc> Cd<jats:sup>2+<\/jats:sup>. L\u2010type channels (sensitive to 2 \u03bc<jats:sc>m<\/jats:sc> nisoldipine) activated at \u201330 mV and carried 21 \u00b1 2% of total <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>. Non\u2010L\u2010type channels activated at potentials positive to \u201310 mV and carried: N channels (sensitive to 1 \u03bc<jats:sc>m<\/jats:sc>\u03c9\u2010conotoxin\u2010GVIA) 16 \u00b1 1% of total <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>, P\/Q channels (sensitive to 3 \u03bc<jats:sc>m<\/jats:sc>\u03c9\u2010conotoxin\u2010MVIIC after nisoldipine plus GVIA) 23 \u00b1 3% of total <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub> and R channels (resistant to all blockers combined) 40 \u00b1 3% of total <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>. CA release induced by hypoxia, hypercapnic acidosis, dinitrophenol (DNP) and high K<jats:sup>+<\/jats:sup><jats:sub>o<\/jats:sub> in the intact CB was inhibited by 79\u201398% by 200 \u03bc<jats:sc>m<\/jats:sc> Cd<jats:sup>2+<\/jats:sup>. Hypoxia, hypercapnic acidosis and DNP, depolarized chemoreceptor cells and eventually generated repetitive action potential discharge. Nisoldipine plus MVIIC nearly abolished the release of CAs induced by hypoxia and hypercapnic acidosis and reduced by 74% that induced by DNP. All these secretory responses were insensitive to GVIA. 30 and 100 m<jats:sc>m<\/jats:sc> K<jats:sup>+<\/jats:sup><jats:sub>o<\/jats:sub> brought resting membrane potential (<jats:italic>E<\/jats:italic><jats:sub>m<\/jats:sub>) of chemoreceptor cells (\u201348.1 \u00b1 1.2 mV) to \u201322.5 and +7.2 mV, respectively. Thirty millimolar K<jats:sup>+<\/jats:sup><jats:sub>o<\/jats:sub>\u2010evoked release was abolished by nisoldipine but that induced by 100 m<jats:sc>m<\/jats:sc> K<jats:sup>+<\/jats:sup><jats:sub>o<\/jats:sub> was mediated by activation of L, N, and P\/Q channels. Data show that tested stimuli depolarize rabbit CB chemoreceptor cells and elicit CA release through Ca<jats:sup>2+<\/jats:sup> entry via voltage\u2010activated channels. Only L and P\/Q channels are tightly coupled to the secretion of CA.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.075523","type":"journal-article","created":{"date-parts":[[2004,11,5]],"date-time":"2004-11-05T01:13:42Z","timestamp":1099617222000},"page":"407-420","source":"Crossref","is-referenced-by-count":32,"title":["Role of voltage\u2010dependent calcium channels in stimulus\u2013secretion coupling in rabbit carotid body chemoreceptor cells"],"prefix":"10.1113","volume":"562","author":[{"given":"Asunci\u00f3n","family":"Rocher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Geijo\u2010Barrientos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Isabel","family":"C\u00e1ceres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Rigual","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constancio","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Almaraz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.058818"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-22-08323.2000"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016254"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.8.2518"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/367072a0"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb04462.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021890"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020143"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020239"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.819ba.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00135.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)00987-0"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019115"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(88)90146-7"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2628046100"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014441"},{"key":"e_1_2_5_18_1","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1152\/jn.1993.70.4.1378","article-title":"L\u2010type calcium channels in type I cells of the rat carotid body","volume":"70","author":"Fieber LA","year":"1993","journal-title":"J Neurophysiol"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10190"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1994.74.4.829"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1997.sp004036"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90221-X"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"H2164","DOI":"10.1152\/ajpheart.1997.272.5.H2164","article-title":"Inhibition by external Cd2+ of Na\/Ca exchange and L\u2010type Ca channel in rabbit ventricular myocytes","volume":"272","author":"Hobai IA","year":"1997","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.2.H692"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.2.C423"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2003.09.075"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)03272-8"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.57.193"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050541"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00696-2"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2456613"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.93.5.1001"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.20028560"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-08-02612.1996"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00003-4"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90223-Z"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.107.1.133"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00006-3"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(92)90260-9"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.004135"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2003.00789.x"},{"key":"e_1_2_5_42_1","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1152\/jn.1997.78.5.2467","article-title":"Ca2+ current in rabbit carotid body glomus cells is conducted by multiple types of high\u2010voltage\u2010activated Ca2+ channels","volume":"78","author":"Overholt JL","year":"1997","journal-title":"J Neurophysiol"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)00832-3"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/rr51"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-04-02995.1995"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018441"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00212-9"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-9280-2_11"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1999.00470.x"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018442"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(89)90125-0"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp021083"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.84.3.1636"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2002.88.2.604"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199508000-00022"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-01-00171.2000"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.21.10208"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.1.C51"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.79.2.622"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(99)00025-8"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(02)02247-6"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-23-08566.2000"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2004.075523","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2004.075523","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T22:03:03Z","timestamp":1697493783000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2004.075523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1,7]]},"references-count":61,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,1,15]]}},"alternative-id":["10.1113\/jphysiol.2004.075523"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2004.075523","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1,7]]}}}