{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T22:03:03Z","timestamp":1791064983421,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T00:00:00Z","timestamp":1106179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p>Treatment of human epithelial kidney (HEK293) cells with low concentrations of the muscarinic agonist methacholine results in the activation of complex and repetitive cycling of intracellular calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), known as [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations. These oscillations occur with a frequency that depends on the concentration of methacholine, whereas the magnitude of the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> spikes does not. The oscillations do not persist in the absence of extracellular Ca<jats:sup>2+<\/jats:sup>, leading to the conclusion that entry of Ca<jats:sup>2+<\/jats:sup> across the plasma membrane plays a significant role in either their initiation or maintenance. However, treatment of cells with high concentrations of GdCl<jats:sub>3<\/jats:sub>, a condition which limits the flux of calcium ions across the plasma membrane in both directions, allows sustained [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations to occur. This suggests that the mechanisms that both initiate and regenerate [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations are intrinsic to the intracellular milieu and do not require entry of extracellular Ca<jats:sup>2+<\/jats:sup>. This would additionally suggest that, under normal conditions, the role of calcium entry is to sustain [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations. By utilizing relatively specific pharmacological manoeuvres we provide evidence that the Ca<jats:sup>2+<\/jats:sup> entry that supports Ca<jats:sup>2+<\/jats:sup> oscillations occurs through the store\u2010operated or capacitative calcium entry pathway. However, by artificial introduction of a non\u2010store\u2010operated pathway into the cells (TRPC3 channels), we find that other Ca<jats:sup>2+<\/jats:sup> entry mechanisms can influence oscillation frequency in addition to the store\u2010operated channels.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.077289","type":"journal-article","created":{"date-parts":[[2004,10,29]],"date-time":"2004-10-29T00:14:29Z","timestamp":1099008869000},"page":"697-706","source":"Crossref","is-referenced-by-count":116,"title":["Capacitative calcium entry supports calcium oscillations in human embryonic kidney cells"],"prefix":"10.1113","volume":"562","author":[{"given":"Gary St. J.","family":"Bird","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"James W.","family":"Putney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,20]]},"reference":[{"key":"e_1_2_3_2_1","first-page":"211","article-title":"Inositol trisphosphate and calcium oscillations","volume":"26","author":"Berridge MJ","year":"1992","journal-title":"Adv Second Messenger Phosphoprotein Res"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/361315a0"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3120001"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021927"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524043360681"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011571200"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020742"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.55.030193.002235"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.31.19554"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2840243"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007524200"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a021780"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80282-2"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.2000.0108"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(86)90026-6"},{"key":"e_1_2_3_17_1","first-page":"84","article-title":"Pharmacology of capacitative calcium entry","volume":"1","author":"Putney JW","year":"2001","journal-title":"Mol Interventions"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.12.2223"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.35.21522"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1054\/ceca.1999.0022"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0310916"},{"key":"e_1_2_3_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3130815"},{"key":"e_1_2_3_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3160819"},{"key":"e_1_2_3_24_1","first-page":"1392","volume-title":"Proc Natl Acad Sci U S A","author":"Sneyd J","year":"2004"},{"key":"e_1_2_3_25_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.10.13.8940296"},{"key":"e_1_2_3_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300544200"},{"key":"e_1_2_3_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202549200"},{"key":"e_1_2_3_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.30.1.44"},{"key":"e_1_2_3_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/319600a0"},{"key":"e_1_2_3_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(87)90038-8"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2004.077289","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2004.077289","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T15:50:18Z","timestamp":1696953018000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2004.077289"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1,20]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["10.1113\/jphysiol.2004.077289"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2004.077289","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1,20]]}}}