{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T13:17:06Z","timestamp":1776604626473,"version":"3.51.2"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,6,13]],"date-time":"2005-06-13T00:00:00Z","timestamp":1118620800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p>Measurements were made (using fast confocal microscopy) of intracellular Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> levels in fluo\u20104 loaded interstitial cells isolated from the rabbit urethra. These cells exhibited regular Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> oscillations which were associated with spontaneous transient inward currents recorded under voltage clamp. Interference with <jats:sc>d<\/jats:sc>\u2010<jats:italic>myo<\/jats:italic>\u2010inositol 1,4,5\u2010trisphosphate (IP<jats:sub>3<\/jats:sub>) induced Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> release using 100 \u03bc<jats:sc>m<\/jats:sc> 2\u2010aminoethoxydiphenyl borate, and the phospholipase C (PLC) inhibitors 2\u2010nitro\u20104\u2010carboxyphenyl <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010diphenylcarbamate and U73122 decreased the amplitude of spontaneous oscillations but did not abolish them. However, oscillations were abolished when ryanodine receptors were blocked with tetracaine or ryanodine. Oscillations ceased in the absence of external Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>, and frequency was directly proportional to the external Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> concentration. Frequency of Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> oscillation was reduced by SKF\u201096365, but not by nifedipine. Lanthanum and cadmium completely blocked oscillations. These results suggest that Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> oscillations in isolated rabbit urethral interstitial cells are initiated by Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> release from ryanodine\u2010sensitive intracellular stores, that oscillation frequency is very sensitive to the external Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> concentration and that conversion of the primary oscillation to a propagated Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> wave depends upon IP<jats:sub>3<\/jats:sub>\u2010induced Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup> release.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.078097","type":"journal-article","created":{"date-parts":[[2005,3,11]],"date-time":"2005-03-11T02:45:03Z","timestamp":1110509103000},"page":"449-461","source":"Crossref","is-referenced-by-count":67,"title":["Calcium oscillations in interstitial cells of the rabbit urethra"],"prefix":"10.1113","volume":"565","author":[{"given":"L.","family":"Johnston","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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