{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T18:30:40Z","timestamp":1718649040863},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,22]],"date-time":"2005-03-22T00:00:00Z","timestamp":1111449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>The influence of extracellular hypotonicity on the relationship between cell volume (<jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub>) and resting membrane potential (<jats:italic>E<\/jats:italic><jats:sub>m<\/jats:sub>) was investigated in <jats:italic>Rana temporaria<\/jats:italic> skeletal muscle. <jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub> was measured by confocal microscope imaging of fibres through their transverse (<jats:italic>xz<\/jats:italic>) planes, and <jats:italic>E<\/jats:italic><jats:sub>m<\/jats:sub> was determined using standard microelectrode techniques. Hypotonic solutions first elicited a rapid increase in fibre volume, \u0394<jats:italic>V<\/jats:italic><jats:sub>R+<\/jats:sub> that fulfilled expectations of simple osmotic behaviour described in earlier reports. However, this was consistently followed by a slow increase in <jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub> (\u0394<jats:italic>V<\/jats:italic><jats:sub>S+<\/jats:sub>) to 10\u201315% above osmotic predictions. Longer (&gt;1 h) exposures to hypotonic solutions permitted a subsequent slow decrease in <jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub> (\u0394<jats:italic>V<\/jats:italic><jats:sub>S\u2212<\/jats:sub>), the eventual magnitude of which exceeded that of the preceding \u0394<jats:italic>V<\/jats:italic><jats:sub>S+<\/jats:sub>. Restoration of isotonic conditions elicited a prompt recovery in <jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub> that matched simple osmotic predictions and thus left a net change in <jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub>. Such alterations in <jats:italic>V<\/jats:italic><jats:sub>c<\/jats:sub> attributable to \u0394<jats:italic>V<\/jats:italic><jats:sub>S+<\/jats:sub> then gradually reversed, while those due to \u0394<jats:italic>V<\/jats:italic><jats:sub>S\u2212<\/jats:sub> persisted. Both \u0394<jats:italic>V<\/jats:italic><jats:sub>S+<\/jats:sub> and \u0394<jats:italic>V<\/jats:italic><jats:sub>S\u2212<\/jats:sub> persisted under conditions of Cl<jats:sup>\u2212<\/jats:sup> deprivation. The depolarization of <jats:italic>E<\/jats:italic><jats:sub>m<\/jats:sub> that accompanied \u0394<jats:italic>V<\/jats:italic><jats:sub>R+<\/jats:sub> was consistent with dilution of intracellular [K<jats:sup>+<\/jats:sup>]. <jats:italic>E<\/jats:italic><jats:sub>m<\/jats:sub> did not significantly alter during the subsequent \u0394<jats:italic>V<\/jats:italic><jats:sub>S<\/jats:sub> transients. These empirical features of \u0394<jats:italic>V<\/jats:italic><jats:sub>S+<\/jats:sub> and \u0394<jats:italic>V<\/jats:italic><jats:sub>S\u2212<\/jats:sub> were analysed using the quantitative charge\u2010difference model of Fraser and Huang, published in 2004. This attributed the \u0394<jats:italic>V<\/jats:italic><jats:sub>S+<\/jats:sub> to an electroneutral increase in the effective osmotic activity of normally membrane\u2010impermeant intracellular anions. In contrast, the \u0394<jats:italic>V<\/jats:italic><jats:sub>S\u2212<\/jats:sub> could only be explained by an efflux of such anions and was accordingly comparable to organic anion\u2010dependent regulatory volume decreases reported in other cell types.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.080911","type":"journal-article","created":{"date-parts":[[2005,1,14]],"date-time":"2005-01-14T01:18:39Z","timestamp":1105665519000},"page":"51-63","source":"Crossref","is-referenced-by-count":16,"title":["Slow volume transients in amphibian skeletal muscle fibres studied in hypotonic solutions"],"prefix":"10.1113","volume":"564","author":[{"given":"James A.","family":"Fraser","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine E. J.","family":"Rang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliet A.","family":"Usher\u2010Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher L.\u2010H.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1956.sp005615"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1965.sp007574"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1707"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-004-2767-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1963.sp007258"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.058545"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.065706"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0987fje"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018670025321"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013298"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.15.10661"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1959.sp006278"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00126436"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.1.247"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000016357"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01872739"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027356410698"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(96)00061-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0003g.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.5.C995"},{"key":"e_1_2_6_22_1","first-page":"38","article-title":"Osmotical behaviour of isolated muscle fibres","volume":"15","author":"Paris M","year":"1965","journal-title":"Acta Physiol Latino Am"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.25.3.209"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.142.3589.246"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.1.211"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.4.1411"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1985.248.2.R190"},{"key":"e_1_2_6_28_1","first-page":"999","article-title":"Morphometry of the human lung: the state of the art after two decades","volume":"15","author":"Weibel ER","year":"1979","journal-title":"Bull Eur Physiopathol Respir"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1999.277.1.R154"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2004.080911","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2004.080911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T02:34:40Z","timestamp":1697250880000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2004.080911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,22]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1113\/jphysiol.2004.080911"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2004.080911","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,22]]}}}