{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:06:33Z","timestamp":1757779593270},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,5,11]],"date-time":"2005-05-11T00:00:00Z","timestamp":1115769600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p>The excitability of smooth muscles is regulated, in part, by background K<jats:sup>+<\/jats:sup> conductances that determine resting membrane potential. However, the K<jats:sup>+<\/jats:sup> conductances so far described in gastrointestinal (GI) muscles are not sufficient to explain the negative resting potentials of these cells. Here we describe expression of two\u2010pore K<jats:sup>+<\/jats:sup> channels of the TASK family in murine small and large intestinal muscles. TASK\u20102, cloned from murine intestinal muscles, resulted in a pH\u2010sensitive, time\u2010dependent, non\u2010inactivating K<jats:sup>+<\/jats:sup> conductance with slow activation kinetics. A similar conductance was found in native intestinal myocytes using whole\u2010cell patch\u2010clamp conditions. The pH\u2010sensitive current was blocked by local anaesthetics. Lidocaine, bupivacaine and acidic pH depolarized circular muscle cells in intact muscles and decreased amplitude and frequency of slow waves. The effects of lidocaine were not blocked by tetraethylammonium chloride, 4\u2010aminopyridine, glibenclamide, apamin or MK\u2010499. However, depolarization by acidic pH was abolished by pre\u2010treatment with lidocaine, suggesting that lidocaine\u2010sensitive K<jats:sup>+<\/jats:sup> channels were responsible for pH\u2010sensitive changes in membrane potential. The kinetics of activation, sensitivity to pH, and pharmacology of the conductance in intestinal myocytes and the expression of TASK\u20101 and TASK\u20102 in these cells suggest that the pH\u2010sensitive background conductance is encoded by TASK genes. This conductance appears to contribute significantly to resting potential and may regulate excitability of GI muscles.<\/jats:p>","DOI":"10.1113\/jphysiol.2005.084574","type":"journal-article","created":{"date-parts":[[2005,3,18]],"date-time":"2005-03-18T04:47:48Z","timestamp":1111121268000},"page":"243-259","source":"Crossref","is-referenced-by-count":23,"title":["A pH\u2010sensitive potassium conductance (TASK) and its function in the murine gastrointestinal tract"],"prefix":"10.1113","volume":"565","author":[{"given":"Sang","family":"Yun Cho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Beckett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salah 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