{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:37:04Z","timestamp":1791250624196,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T00:00:00Z","timestamp":1119398400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,6,15]]},"abstract":"<jats:p>In the immature hippocampus, the so\u2010called \u2018giant depolarizing potentials\u2019 (GDPs) are network\u2010driven synaptic events generated by the synergistic action of glutamate and GABA. Here we tested the hypothesis that ATP, a widely distributed neurotransmitter, directly contributes to the network activity during the first postnatal week. We found that in CA3 pyramidal cells, in the presence of the adenosine antagonist 8\u2010cyclopentyl\u20101,3\u2010dipropylxanthine (DPCPX), ATP produced a transient facilitation of GDPs followed by a depressant effect. A similar biphasic effect was produced by blockade of the ectoATPase activity with 6\u2010<jats:italic>N,N<\/jats:italic>\u2010diethyl\u2010<jats:sc>d<\/jats:sc>\u2010\u03b2,\u03b3\u2010dibromomethylene ATP (ARL\u201067156). The effects of exogenous and endogenous ATP on GDPs were prevented by the P2X receptor antagonist pyridoxal phosphate\u20106\u2010azophenyl\u20102\u2032,4\u2032\u2010disulphonic acid (PPADS). On pyramidal cells, ATP upregulated spontaneous action\u2010potential\u2010dependent GABA<jats:sub>A<\/jats:sub>\u2010mediated synaptic events (GABA\u2010SPSPs), suggesting a network\u2010driven effect. Recordings from interneurones allowed comparison of ATP effects on GABAergic and glutamatergic synaptic activity. While ATP depressed GABA\u2010SPSPs <jats:italic>via<\/jats:italic> metabotropic P2Y<jats:sub>1<\/jats:sub> receptors, it up\u2010 and downregulated glutamatergic SPSPs <jats:italic>via<\/jats:italic> PPADS\u2010sensitive receptors. Thus, ATP exerts an excitatory action on CA3 pyramidal cells <jats:italic>via<\/jats:italic> facilitation of GDPs and SPSPs. This excitatory drive is propagated to pyramidal cells by interneurons that represent the \u2018common pathway\u2019 for generation of GDPs and SPSPs. Our results show that ATP operating via distinct P2X and P2Y receptors directly contributes to modulate network activity at the early stages of postnatal development.<\/jats:p>","DOI":"10.1113\/jphysiol.2005.085621","type":"journal-article","created":{"date-parts":[[2005,4,22]],"date-time":"2005-04-22T00:14:26Z","timestamp":1114128866000},"page":"981-992","source":"Crossref","is-referenced-by-count":27,"title":["ATP contributes to the generation of network\u2010driven giant depolarizing potentials in the neonatal rat hippocampus"],"prefix":"10.1113","volume":"565","author":[{"given":"Victoria F.","family":"Safiulina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander 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