{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T08:53:35Z","timestamp":1768035215201,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,7,19]],"date-time":"2005-07-19T00:00:00Z","timestamp":1121731200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p>The carotid body (CB) chemoreceptors participate in the ventilatory responses to acute and chronic hypoxia (CH). Arterial hypoxaemia increases breathing within seconds, and CB chemoreceptors are the principal contributors to this reflex hyperventilatory response. Acute hypoxia induces depolarization of CB chemoreceptors by inhibiting certain K<jats:sup>+<\/jats:sup> channels, but the role of these channels in CH, as in high\u2010altitude acclimatization, is less known. Here we explored the effects of prolonged (24\u201348 h) hypoxic exposure of rabbit CB chemoreceptor cells in primary cultures on the voltage\u2010dependent K<jats:sup>+<\/jats:sup> currents and on their response to acute hypoxia. We found that CH induces a decrease in the amplitude of outward K<jats:sup>+<\/jats:sup> currents due to a reduction in a fast\u2010inactivating BDS\u2010 and highly TEA\u2010sensitive component of the current. In spite of this effect, acute hypoxic inhibition of K<jats:sup>+<\/jats:sup> currents is increased in CH cultures, as well as hypoxia\u2010induced depolarization. These data suggest that downregulation of this component (that does not contribute to the oxygen\u2010sensitive K<jats:sup>+<\/jats:sup> current (<jats:italic>I<\/jats:italic><jats:sub>KO2<\/jats:sub>)) participates in the hypoxic sensitization. Pharmacological, immunocytochemical and quantitative PCR (qPCR) experiments demonstrate that CH\u2010induced decrease in outward K<jats:sup>+<\/jats:sup> currents is due to a downregulation of the expression of Kv3.4 channels. Taken together, our results suggest that CH sensitization in rabbit CB could be achieved by an increase in the relative contribution of <jats:italic>I<\/jats:italic><jats:sub>KO2<\/jats:sub> to the outward K<jats:sup>+<\/jats:sup> current as a consequence of the decreased expression of the oxygen\u2010insensitive component of the current. We conclude that acute and chronic hypoxia can exert their effects acting on different molecular targets.<\/jats:p>","DOI":"10.1113\/jphysiol.2005.085837","type":"journal-article","created":{"date-parts":[[2005,5,13]],"date-time":"2005-05-13T00:14:25Z","timestamp":1115943265000},"page":"395-408","source":"Crossref","is-referenced-by-count":40,"title":["Down regulation of Kv3.4 channels by chronic hypoxia increases acute oxygen sensitivity in rabbit carotid body"],"prefix":"10.1113","volume":"566","author":[{"given":"Stefan","family":"K\u00e4\u00e4b","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Miguel\u2010Velado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Ram\u00f3n","family":"L\u00f3pez\u2010L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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