{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:03:03Z","timestamp":1788382983706,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,8,10]],"date-time":"2005-08-10T00:00:00Z","timestamp":1123632000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p>Lumbar splanchnic (LSN) and sacral pelvic (PN) nerves convey different mechanosensory information from the colon to the spinal cord. Here we determined whether these pathways also differ in their chemosensitivity and receptor expression. Using an <jats:italic>in vitro<\/jats:italic> mouse colon preparation, individual primary afferents were tested with selective P2X and transient receptor potential vanilloid receptor 1 (TRPV1) receptor ligands. Afferent cell bodies in thoracolumbar and lumbosacral dorsal root ganglia (DRG) were retrogradely labelled from the colon and analysed for P2X<jats:sub>3<\/jats:sub>\u2010 and TRPV1\u2010like immunoreactivity (LI). Forty per cent of LSN afferents responded to \u03b1,\u03b2\u2010methylene adenosine 5\u2032\u2010triphosphate (\u03b1,\u03b2\u2010meATP; 1 m<jats:sc>m<\/jats:sc>), an effect that was concentration dependent and reversed by the P2X antagonist pyridoxyl5\u2010phosphate 6\u2010azophenyl\u20102\u2032,4\u2032\u2010disulphonic acid (PPADS) (100 \u03bc<jats:sc>m<\/jats:sc>). Significantly fewer PN afferents (7%) responded to \u03b1,\u03b2\u2010meATP. Correspondingly, 36% of colonic thoracolumbar DRG neurones exhibited P2X<jats:sub>3<\/jats:sub>\u2010LI compared with only 19% of colonic lumbosacral neurones. Capsaicin (3 \u03bc<jats:sc>m<\/jats:sc>) excited 61% of LSN afferents and 47% of PN afferents; 82% of thoracolumbar and 50% of lumbosacral colonic DRG neurones displayed TRPV1\u2010LI. Mechanically insensitive afferents were recruited by \u03b1,\u03b2\u2010meATP or capsaicin, and were almost exclusive to the LSN. Capsaicin\u2010responsive LSN afferents displayed marked mechanical desensitization after responding to capsaicin, which did not occur in capsaicin\u2010responsive PN afferents. Therefore, colonic LSN and PN pathways differ in their chemosensitivity to known noxious stimuli and their corresponding receptor expression. As these pathways relay information that may relate to symptoms in functional gastrointestinal disease, these results may have implications for the efficacy of therapies targeting receptor modulation.<\/jats:p>","DOI":"10.1113\/jphysiol.2005.089714","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T00:13:14Z","timestamp":1118362394000},"page":"267-281","source":"Crossref","is-referenced-by-count":127,"title":["Differential chemosensory function and receptor expression of splanchnic and pelvic colonic afferents in mice"],"prefix":"10.1113","volume":"567","author":[{"given":"Stuart M.","family":"Brierley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Carter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"W.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gareth A.","family":"Hicks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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