{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:40:55Z","timestamp":1787758855687,"version":"build-2784847793"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,4]],"date-time":"2006-01-04T00:00:00Z","timestamp":1136332800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>Non\u2010invasive recordings of the retinal activity have an important role to play in the diagnosis of retinal pathologies. The detection of diseases that involve retinal ganglion cells (RGCs), such as optic atrophy and glaucoma, may be improved by isolating the RGC contribution from the multifocal electroretinogram (mfERG). In this study, mfERGs were performed on 20 pigs, 1\u20136 weeks following unilateral retrobulbar optic nerve section (ONS). The stimuli were 103 non\u2010scaled high\u2010contrast hexagons from which summed and individual mfERG responses were obtained in experimental and control fellow eyes under conditions of ketamine (<jats:italic>n<\/jats:italic>= 11) or isoflurane anaesthesia (<jats:italic>n<\/jats:italic>= 9). The effect of intravitreal injection of tetrodotoxin (TTX; <jats:italic>n<\/jats:italic>= 6) was also investigated. The summed mfERG responses showed a first positive peak (P1) with a short latency (21 ms) followed by two smaller peaks (P2 and P3) of longer latency (46 and 65 ms, respectively). While P2 and P3 amplitude were highly correlated with the time post\u2010optic nerve section (ONS) <jats:styled-content>(P2: <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.669; <jats:italic>P<\/jats:italic>= 0.007; P3: <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.651; <jats:italic>P<\/jats:italic>= 0.005)<\/jats:styled-content>, P1 was not <jats:styled-content>(<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.193; <jats:italic>P<\/jats:italic>= 0.38)<\/jats:styled-content>. P1 and P2 showed no implicit time variation as a function of retinal location, while P3 implicit time varied along the axis of the visual streak, generating a naso\u2010temporal asymmetry. However, the P3 implicit time did not vary consistently with distance away from the optic nerve head. Intravitreal injections of TTX reduced P2 and P3 in the control eyes, consistent with the effect of ONS, and also induced a series of regular oscillations lasting up to 200 ms post stimulus. Under isoflurane anaesthesia, all components of the mfERG ifn experimental and control eyes were, at all time points post\u2010ONS, of similar amplitude and without naso\u2010temporal asymmetry, suggesting a reduced participation of RGCs under these anaesthesic conditions. These data clearly demonstrate that it is possible to isolate the RGC contribution from non\u2010invasive multifocal electroretinography.<\/jats:p>","DOI":"10.1113\/jphysiol.2005.098046","type":"journal-article","created":{"date-parts":[[2005,11,11]],"date-time":"2005-11-11T01:14:26Z","timestamp":1131671666000},"page":"325-338","source":"Crossref","is-referenced-by-count":29,"title":["Retinal ganglion cell activity from the multifocal electroretinogram in pig: optic nerve section, anaesthesia and intravitreal tetrodotoxin"],"prefix":"10.1113","volume":"570","author":[{"given":"M\u00e9lanie R.","family":"Lalonde","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balwantray 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