{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:26:51Z","timestamp":1790670411463,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,3,7]],"date-time":"2006-03-07T00:00:00Z","timestamp":1141689600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>Vascular oxidative stress is the key mechanism involved in the age\u2010related decline in endothelium\u2010dependent dilatation (EDD). We tested the hypothesis that xanthine oxidase (XO), a major vascular source of reactive oxygen species, contributes to the impairment in EDD with ageing. At baseline, brachial artery flow\u2010mediated dilatation (FMD) was 55% lower in older (<jats:italic>n<\/jats:italic>= 9, 64 \u00b1 2 years, 8M\/1F, mean \u00b1<jats:sc>s.e.m.<\/jats:sc>) <jats:italic>versus<\/jats:italic> young (<jats:italic>n<\/jats:italic>= 9, 26 \u00b1 1 years, 8M\/1F) healthy adults (3.41 \u00b1 0.44 <jats:italic>versus<\/jats:italic> 7.53 \u00b1 0.67%, <jats:italic>P<\/jats:italic> &lt; 0.001), whereas endothelium\u2010independent dilatation (EID; sublingual nitroglycerin) did not differ between groups. Plasma oxidized low\u2010density lipoprotein (oxi\u2010LDL), a measure of systemic oxidative stress, was greater at baseline in the older subjects (58.3 \u00b1 5.9 <jats:italic>versus<\/jats:italic> 46.8 \u00b1 2.4 U l<jats:sup>\u22121<\/jats:sup>, <jats:italic>P<\/jats:italic> &lt; 0.05) and inversely correlated with baseline FMD (<jats:italic>r<\/jats:italic>=\u2212 0.54; <jats:italic>P<\/jats:italic> &lt; 0.05). Acute administration of allopurinol, a competitive inhibitor of XO, reduced plasma uric acid concentrations similarly in both groups (<jats:italic>P<\/jats:italic> &lt; 0.001), but did not affect FMD, EID, or oxi\u2010LDL in either group. Vascular endothelial protein expression of XO (immunofluorescence) was not different in antecubital venous cells from the young and older subjects (0.56 \u00b1 0.12 <jats:italic>versus<\/jats:italic> 0.68 \u00b1 0.19 XO intensity\/human umbilical vein endothelial cell intensity, <jats:italic>P<\/jats:italic>= 0.49). We conclude that XO does not contribute to oxidative stress\u2010associated reductions in peripheral conduit artery EDD with ageing in humans, possibly due to an absence of age\u2010associated up\u2010regulation of endothelial XO.<\/jats:p>","DOI":"10.1113\/jphysiol.2005.102566","type":"journal-article","created":{"date-parts":[[2006,1,27]],"date-time":"2006-01-27T01:24:08Z","timestamp":1138325048000},"page":"661-668","source":"Crossref","is-referenced-by-count":81,"title":["Xanthine oxidase does not contribute to impaired peripheral conduit artery endothelium\u2010dependent dilatation with ageing"],"prefix":"10.1113","volume":"571","author":[{"given":"Iratxe","family":"Eskurza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary D.","family":"Kahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas 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